| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL14A1 |
| Uniprot No | Q05707 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-759aa |
| 氨基酸序列 | MDPGALEMKNFNKIISFLYSTVGALNKIGTDGTQVAMVQFTDDPRTEFKLNAYKTKETLLDAIKHISYKGGNTKTGKAIKYVRDTLFTAESGTRRGIPKVIVVITDGRSQDDVNKISREMQLDGYSIFAIGVADADYSELVSIGSKPSARHVFFVDDFDAFKKIEDELITFVCETASATCPVVHKDGIDLAGFKMMEMFGLVEKDFSSVEGVSMEPGTFNVFPCYQLHKDALVSQPTRYLHPEGLPSDYTISFLFRILPDTPQEPFALWEILNKNSDPLVGVILDNGGKTLTYFNYDQSGDFQTLTFEGPEIRKIFYGSFHKLHIVVSETLVKVVIDCKQVGEKAMNASANITSDGVEVLGKMVRSRGPGGNSAPFQLQMFDIVCSTSWANTDKCCELPGLRDDESCPDLPHSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEPGPKGPDGPRGEIGLPGPQGPPGPQGPSGLSIQGMPGMPGEKGEKGDTGLPGPQGIPGGVGSPGRDGSPGQRGLPGKDGSSGPPGPPGPIGIPGTPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRSVARQVCEQLTQSHMARYTAILNQIPSHSSSIRTVQGPPGEPGRPGSPGAPGEQGPPGTPGFPGNAGVPGTPGERGLTGIKGEKGNPGVGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVRAPHPDQPEFTPVQDELEAMELWGPGV |
| 分子量 | 109.23 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COL14A1蛋白的示例参考文献(内容为模拟概括，非真实文献)：
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1. **标题**：**"Expression and Functional Analysis of Recombinant Human COL14A1 in Extracellular Matrix Remodeling"**
**作者**：Smith A, et al. (2022)
**摘要**：本研究在哺乳动物细胞系中成功表达并纯化了重组人COL14A1蛋白，发现其通过调节胶原纤维的组装参与细胞外基质重塑，并在成纤维细胞迁移和黏附中发挥关键作用。
2. **标题**：**"COL14A1 as a Novel Biomarker in Liver Fibrosis: Insights from Recombinant Protein Studies"**
**作者**：Zhang L, et al. (2020)
**摘要**：通过重组COL14A1蛋白的体外实验，发现其与肝星状细胞活化密切相关，并通过抑制TGF-β信号通路减缓纤维化进程，提示其作为肝纤维化治疗的潜在靶点。
3. **标题**：**"Structural Characterization of Recombinant COL14A1 and Its Interaction with Collagen I"**
**作者**：Tanaka K, et al. (2019)
**摘要**：利用重组人COL14A1蛋白进行结构解析，揭示了其N端非胶原结构域与胶原I的特异性结合模式，为理解其在组织机械稳定性中的功能提供分子基础。
4. **标题**：**"Proteolytic Processing of Recombinant COL14A1 in Tumor Stroma and Implications for Cancer Progression"**
**作者**：Chen R, et al. (2021)
**摘要**：研究证实肿瘤微环境中重组COL14A1蛋白被基质金属蛋白酶切割，释放的功能性片段促进血管生成和癌细胞侵袭，提示其在肿瘤微环境中的双重调控作用。
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**说明**：上述文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以关键词“recombinant COL14A1”“collagen XIV expression”等检索真实文献。
Recombinant human COL14A1 protein is derived from the COL14A1 gene, which encodes the alpha-1 chain of type XIV collagen, a member of the fibril-associated collagens with interrupted triple helices (FACIT) family. Type XIV collagen plays a critical role in regulating the organization and stability of extracellular matrices (ECMs), particularly in tissues subjected to mechanical stress, such as skin, blood vessels, and tendons. It interacts with other ECM components, including fibrillar collagens (e.g., types I and III) and proteoglycans, to modulate fibrillogenesis, cell adhesion, and tissue remodeling. COL14A1 contains multiple functional domains, such as von Willebrand factor A-like domains and a fibrillar collagen domain, enabling its role as a molecular bridge in ECM networks.
Recombinant COL14A1 is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, including disulfide bond formation and glycosylation. This protein is widely utilized in biomedical research to study ECM assembly, cell-matrix interactions, and pathological conditions like fibrosis, cancer metastasis, and cardiovascular diseases. Abnormal COL14A1 expression has been linked to tissue stiffness in tumors and impaired wound healing. Its recombinant form serves as a tool for developing therapeutic strategies targeting ECM dysregulation. Purified recombinant COL14A1 is characterized by techniques such as SDS-PAGE, Western blotting, and functional assays to validate its bioactivity in modulating cellular behavior and matrix remodeling.
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