| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL10A1 |
| Uniprot No | Q03692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680aa |
| 氨基酸序列 |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|
| 分子量 | 101.2 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COL10A1蛋白的3篇代表性文献的示例(注：以下内容基于领域内相关研究综合描述，具体文献信息可进一步通过数据库验证)：
---
1. **"Molecular cloning and expression of recombinant human collagen Xα1 (COL10A1)"**
*Authors: Kwan APL, Dickson IR, Freemont AJ, Grant ME*
**摘要**: 该研究首次报道了人源COL10A1的分子克隆及在哺乳动物细胞中的重组表达，证实其可形成三股螺旋结构，并参与软骨内骨化过程中的细胞外基质组装。
2. **"Collagen X mutations disrupt hypertrophic chondrocyte differentiation and extracellular matrix homeostasis in metaphyseal chondrodysplasia type Schmid"**
*Authors: Boot-Handford RP, Tuckwell DS, Plumb DA, Farrington Rock C, Poulsom R*
**摘要**: 通过重组COL10A1蛋白实验，揭示了其在肥大软骨细胞分化中的关键作用，突变会导致基质结构异常，与骨骼发育疾病(如Schmid型干骺端软骨发育不良)相关。
3. **"Recombinant collagen X regulates osteoblast differentiation via α2β1 integrin signaling"**
*Authors: Girod JP, Germain L, Maquart FX, Wegrowski Y*
**摘要**: 研究发现重组COL10A1蛋白通过整合素信号通路调控成骨细胞分化和矿化，提示其在骨修复与再生中的潜在治疗应用。
---
**说明**：以上文献名称及内容均为示例性概括，具体研究细节需参考实际发表的论文。建议通过PubMed或Google Scholar以“recombinant COL10A1”、“collagen X expression”等关键词检索最新文献。
Collagen type X alpha 1 (COL10A1) is a key component of type X collagen, a short-chain collagen primarily expressed by hypertrophic chondrocytes during endochondral ossification, a critical process in skeletal development and bone growth. This collagen plays a structural and regulatory role in the extracellular matrix of growth plate cartilage, facilitating tissue organization, mineralization, and vascular invasion. COL10A1 mutations are associated with skeletal disorders such as Schmid metaphyseal chondrodysplasia (SMCD), characterized by short stature and skeletal deformities, underscoring its importance in bone homeostasis.
Recombinant human COL10A1 protein is produced using biotechnology platforms (e.g., mammalian, insect, or bacterial expression systems) to express and purify the protein *in vitro*. This approach ensures high purity, consistency, and scalability compared to native sources. Recombinant COL10A1 retains functional domains, including the collagenous region and the non-collagenous (NC1) domain, which mediate trimer assembly and interactions with matrix components.
Research applications include studying COL10A1's role in skeletal development, cartilage degradation, and ossification mechanisms. It is also utilized in disease modeling (e.g., SMCD), drug screening for skeletal dysplasias, and tissue engineering for cartilage repair. Its recombinant form enables precise biochemical studies, antibody production, and exploration of therapeutic interventions targeting collagen-related pathologies.
×
