| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COG2 |
| Uniprot No | Q14746 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-738aa |
| 氨基酸序列 | MEKSRMNLPKGPDTLCFDKDEFMKEDFDVDHFVSDCRKRVQLEELRDDLELYYKLLKTAMVELINKDYADFVNLSTNLVGMDKALNQLSVPLGQLREEVLSLRSSVSEGIRAVDERMSKQEDIRKKKMCVLRLIQVIRSVEKIEKILNSQSSKETSALEASSPLLTGQILERIATEFNQLQFHAVQSKGMPLLDKVRPRIAGITAMLQQSLEGLLLEGLQTSDVDIIRHCLRTYATIDKTRDAEALVGQVLVKPYIDEVIIEQFVESHPNGLQVMYNKLLEFVPHHCRLLREVTGGAISSEKGNTVPGYDFLVNSVWPQIVQGLEEKLPSLFNPGNPDAFHEKYTISMDFVRRLERQCGSQASVKRLRAHPAYHSFNKKWNLPVYFQIRFREIAGSLEAALTDVLEDAPAESPYCLLASHRTWSSLRRCWSDEMFLPLLVHRLWRLTLQILARYSVFVNELSLRPISNESPKEIKKPLVTGSKEPSITQGNTEDQGSGPSETKPVVSISRTQLVYVVADLDKLQEQLPELLEIIKPKLEMIGFKNFSSISAALEDSQSSFSACVPSLSSKIIQDLSDSCFGFLKSALEVPRLYRRTNKEVPTTASSYVDSALKPLFQLQSGHKDKLKQAIIQQWLEGTLSESTHKYYETVSDVLNSVKKMEESLKRLKQARKTTPANPVGPSGGMSDDDKIRLQLALDVEYLGEQIQKLGLQASDIKSFSALAELVAAAKDQATAEQP |
| 分子量 | 107.58 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COG2蛋白的模拟参考文献示例，基于该领域常见研究方向整理而成：
1. **文献名称**：**"Expression and Functional Characterization of Recombinant Human COG2 in Golgi Complex Assembly"**
**作者**：Ungar D, Oka T, Krieger M
**摘要**：研究通过重组技术表达人源COG2蛋白，揭示其在维持高尔基体完整性和COG复合体组装中的关键作用，并验证其与COG1亚基的相互作用。
2. **文献名称**：**"Role of COG2 in Glycosylation Enzyme Trafficking: Insights from Recombinant Protein Reconstitution"**
**作者**：VanRheenen SM, Cao X, Lupashin VV
**摘要**：利用重组COG2蛋白进行体外功能实验，证明COG2缺失导致高尔基体糖基化酶定位异常，进而影响糖蛋白合成。
3. **文献名称**：**"Optimization of Recombinant COG2 Protein Production in Escherichia coli for Structural Studies"**
**作者**：Smith JL, Zhang Y, Li H
**摘要**：开发高效原核表达系统生产可溶性重组人COG2蛋白，优化纯化流程并通过质谱验证蛋白完整性，为后续晶体学研究奠定基础。
4. **文献名称**：**"COG2 Mutations in Congenital Disorders of Glycosylation: Functional Rescue by Recombinant Wild-Type Protein"**
**作者**：Foulger R, Steet RA, Kornfeld S
**摘要**：通过构建致病性COG2突变体，结合重组野生型蛋白的补充实验，阐明特定突变导致细胞内运输缺陷的分子机制。
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**注**：以上文献为示例性整理，实际研究中需根据具体课题检索真实发表的文献(建议使用PubMed、Web of Science等数据库，结合关键词“COG2”“recombinant protein”“Golgi trafficking”等)。
**Background of Recombinant Human COG2 Protein**
The Conserved Oligomeric Golgi (COG) complex is a critical multi-subunit machinery involved in maintaining Golgi architecture, vesicular trafficking, and glycosylation processes. COG2. a key subunit of this complex, plays an essential role in tethering vesicles during intra-Golgi transport and ensuring proper protein sorting and modification. Dysfunction of COG2 is linked to congenital disorders of glycosylation (CDGs), neurological abnormalities, and impaired cellular secretion.
Recombinant human COG2 protein is engineered using heterologous expression systems (e.g., mammalian or bacterial cells) to enable functional studies. Its production facilitates research into Golgi-associated pathways, including mechanisms of vesicle-mediated transport, glycosylation defects, and disease pathogenesis. Structural and biochemical analyses of recombinant COG2 have provided insights into its interactions with other COG subunits (e.g., COG1. COG3-8) and Rab GTPases, which coordinate membrane fusion events.
Studies utilizing recombinant COG2 have also explored its role in cancer cell proliferation and immune regulation, highlighting its broader therapeutic potential. By elucidating COG2's molecular functions, researchers aim to develop targeted interventions for COG-related disorders and advance applications in biotechnology and medicine.
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