| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CMPK |
| Uniprot No | P30085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-196aa |
| 氨基酸序列 | MKPLVVFVLG GPGAGKGTQC ARIVEKYGYT HLSAGELLRD ERKNPDSQYG ELIEKYIKEG KIVPVEITIS LLKREMDQTM AANAQKNKFL IDGFPRNQDN LQGWNKTMDG KADVSFVLFF DCNNEICIER CLERGKSSGR SDDNRESLEK RIQTYLQSTK PIIDLYEEMG KVKKIDASKS VDEVFDEVVQ IFDKEG |
| 分子量 | 22.2 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CMPK蛋白的示例参考文献(注：文献信息为示例，需通过学术数据库验证具体内容)：
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1. **文献名称**：*Mitochondrial CMPK2 mediates antiviral innate immunity by regulating nucleotide metabolism*
**作者**：Li X, et al.
**摘要**：本研究揭示了线粒体CMPK2在抗病毒天然免疫中的关键作用。通过重组表达人CMPK2蛋白，发现其通过调控核苷酸代谢阻止病毒双链RNA(dsRNA)的异常积累，从而增强宿主细胞的I型干扰素反应。
2. **文献名称**：*Structural and functional analysis of human CMPK2 reveals a conserved kinase mechanism*
**作者**：Zhang Y, et al.
**摘要**：报道了重组人CMPK2蛋白的晶体结构解析，阐明了其底物结合域和催化活性位点的特征。通过体外酶活实验，证实CMPK2在CTP合成中的高效催化功能，为靶向核苷酸代谢的药物设计提供依据。
3. **文献名称**：*Recombinant production and biochemical characterization of human CMPK2 in Escherichia coli*
**作者**：Wang H, et al.
**摘要**：成功利用大肠杆菌表达系统实现重组人CMPK2的高效可溶性表达，并建立纯化流程。酶动力学研究表明，重组蛋白具有与天然蛋白相似的催化活性，为后续功能研究奠定基础。
4. **文献名称**：*CMPK2 deficiency inhibits cell proliferation via mitochondria dysfunction in cancer cells*
**作者**：Chen L, et al.
**摘要**：通过重组人CMPK2功能回补实验，证明CMPK2缺失导致线粒体核苷酸代谢紊乱，进而抑制肿瘤细胞增殖。研究强调了CMPK2在癌症代谢中的潜在治疗意义。
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**提示**：建议通过 **PubMed、Web of Science** 或 **Google Scholar** 输入关键词“recombinant human CMPK2”或“CMPK2 protein function”获取真实文献。实际研究中，CMPK2常与线粒体DNA合成、抗病毒免疫及癌症代谢相关。
Recombinant human CMPK (cytidine/uridine monophosphate kinase) protein is a key enzyme involved in nucleotide metabolism, particularly in the biosynthesis of pyrimidine nucleotides essential for DNA and RNA synthesis. CMPK catalyzes the phosphorylation of cytidine monophosphate (CMP) and uridine monophosphate (UMP) to their respective diphosphate forms (CDP and UDP), which are further processed to triphosphates (CTP and UTP) for nucleic acid synthesis and other cellular processes. Two isoforms exist in humans: CMPK1 (cytosolic) and CMPK2 (mitochondrial). CMPK2 is critical for mitochondrial DNA replication, as it supports the generation of deoxythymidine triphosphate (dTTP) via the thymidylate salvage pathway.
The recombinant form of CMPK is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems to ensure proper folding and enzymatic activity. Its study provides insights into nucleotide homeostasis, mitochondrial dysfunction, and diseases like cancer or viral infections, where pyrimidine metabolism is dysregulated. Recombinant CMPK is also utilized in drug discovery, serving as a target for inhibitors aimed at disrupting pathogen or cancer cell proliferation. Additionally, it aids in structural biology studies to elucidate substrate specificity and catalytic mechanisms. Research on CMPK has gained momentum due to its role in metabolic reprogramming in rapidly dividing cells, making it a potential biomarker or therapeutic candidate in oncology and infectious diseases.
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