| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CMPK2 |
| Uniprot No | Q5EBM0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 151-250aa |
| 氨基酸序列 | GACQEAPRPHLGEFEADPRGQLWQRLWEVQDGRRLQVGCAQVVPVPEPPLHPVVPDLPSSVVFPDREAARAVLEECTSFIPEARAVLDLVDQCPKQIQKG |
| 分子量 | 36.74 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CMPK2蛋白的参考文献概要：
1. **文献名称**：*CMPK2 is a mitochondrial UMP-CMP kinase that supports homeostasis of mitochondrial respiratory chain*
**作者**：Lee, H., et al.
**摘要**：研究发现CMPK2定位于线粒体，通过催化UMP/CMP生成UTP/CTP，维持线粒体RNA转录和呼吸链复合体稳定，缺失会导致线粒体功能缺陷。
2. **文献名称**：*CMPK2 is an innate immune regulator of HIV infection through antagonizing SAMHD1*
**作者**：Zhang, C., et al.
**摘要**：揭示CMPK2在HIV感染中的双重作用：受干扰素诱导上调，一方面通过限制dNTP合成抑制病毒复制，另一方面拮抗SAMHD1蛋白促进固有免疫应答。
3. **文献名称**：*Mitochondrial CMPK2 mediates immune modulatory and anticancer activities of IFN-α*
**作者**：Wang, Y., et al.
**摘要**：证明干扰素α通过激活CMPK2调控线粒体代谢重编程，促进氧化磷酸化并抑制肿瘤生长，CMPK2缺失削弱IFN-α的抗癌效应。
**备注**：以上文献及内容为示例性质，实际检索时建议通过PubMed或Web of Science结合关键词(如“CMPK2 recombinant protein”“human CMPK2 function”)获取最新研究。
Recombinant human CMPK2 (cytidine monophosphate kinase 2) is a protein encoded by the CMPK2 gene located on chromosome 2. As a member of the nucleoside monophosphate kinase family, CMPK2 catalyzes the phosphorylation of nucleoside monophosphates like UMP to their diphosphate forms (e.g., UDP), playing a critical role in nucleotide metabolism and maintaining cellular nucleotide pools. It is structurally characterized by a conserved kinase domain and mitochondrial localization signals, suggesting its involvement in mitochondrial DNA synthesis and energy metabolism.
CMPK2 has garnered attention for its roles in diverse biological processes, including immune response modulation, viral infection resistance, and cellular apoptosis. Studies indicate it is upregulated during interferon signaling, linking it to antiviral defense mechanisms. Dysregulation of CMPK2 has been implicated in pathologies such as cancer, neuroinflammatory disorders, and mitochondrial dysfunction.
Recombinant CMPK2 is produced using expression systems like *E. coli* or mammalian cells, enabling high-purity yields for functional studies. Its applications span enzymatic activity assays, structural biology (e.g., crystallography), and screening for inhibitors or activators as potential therapeutics. In cancer research, targeting CMPK2 has been explored due to its dual role in promoting tumorigenesis (via nucleotide synthesis) and apoptosis, depending on cellular context. Continued research aims to clarify its precise mechanisms and therapeutic potential in diseases linked to nucleotide imbalance or mitochondrial dysfunction.
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