| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CMIP |
| Uniprot No | Q8IY22 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-773aa |
| 氨基酸序列 | MDVTSSSGGG GDPRQIEETK PLLGGDVSAP EGTKMGAVPC RRALLLCNGM RYKLLQEGDI QVCVIRHPRT FLSKILTSKF LRRWEPHHLT LADNSLASAT PTGYMENSVS YSAIEDVQLL SWENAPKYCL QLTIPGGTVL LQAANSYLRD QWFHSLQWKK KIYKYKKVLS NPSRWEVVLK EIRTLVDMAL TSPLQDDSIN QAPLEIVSKL LSENTNLTTQ EHENIIVAIA PLLENNHPPP DLCEFFCKHC RERPRSMVVI EVFTPVVQRI LKHNMDFGKC PRLRLFTQEY ILALNELNAG MEVVKKFIQS MHGPTGHCPH PRVLPNLVAV CLAAIYSCYE EFINSRDNSP SLKEIRNGCQ QPCDRKPTLP LRLLHPSPDL VSQEATLSEA RLKSVVVASS EIHVEVERTS TAKPALTASA GNDSEPNLID CLMVSPACST MSIELGPQAD RTLGCYVEIL KLLSDYDDWR PSLASLLQPI PFPKEALAHE KFTKELKYVI QRFAEDPRQE VHSCLLSVRA GKDGWFQLYS PGGVACDDDG ELFASMVHIL MGSCYKTKKF LLSLAENKLG PCMLLALRGN QTMVEILCLM LEYNIIDNND TQLQIISTLE STDVGKRMYE QLCDRQRELK ELQRKGGPTR LTLPSKSTDA DLARLLSSGS FGNLENLSLA FTNVTSACAE HLIKLPSLKQ LNLWSTQFGD AGLRLLSEHL TMLQVLNLCE TPVTDAGLLA LSSMKSLCSL NMNSTKLSAD TYEDLKAKLP NLKEVDVRYT EAW |
| 分子量 | 86.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4篇假设性关于重组人CMIP蛋白的参考文献，供参考：
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1. **文献名称**：*Cloning and Expression of Recombinant Human CMIP Protein in E. coli*
**作者**：Zhang Y, et al.
**摘要**：该研究成功克隆了人源CMIP基因，并在大肠杆菌中实现了重组蛋白的高效表达。通过优化纯化条件获得高纯度CMIP蛋白，验证了其与足细胞骨架蛋白的体外相互作用，为后续功能研究奠定基础。
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2. **文献名称**：*CMIP Modulates T-Cell Signaling Pathways via Interaction with Lck Kinase*
**作者**：Smith J, Patel R.
**摘要**：本文利用重组人CMIP蛋白进行免疫共沉淀实验，发现CMIP通过与T细胞受体信号通路中的Lck激酶结合，负调控T细胞活化，提示其在自身免疫性疾病中的潜在作用。
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3. **文献名称**：*Functional Characterization of Recombinant CMIP in Podocyte Dysfunction*
**作者**：Li X, Wang H.
**摘要**：研究证实，重组CMIP蛋白过表达可导致足细胞骨架重组和凋亡，揭示了CMIP在肾病综合征中的病理机制，并提出其作为治疗靶点的可能性。
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4. **文献名称**：*Structural Insights into CMIP Protein by Cryo-EM Analysis*
**作者**：Garcia S, et al.
**摘要**：通过冷冻电镜解析重组人CMIP蛋白的三维结构，发现其包含独特的α-螺旋结构域，可能参与蛋白质间相互作用，为开发小分子抑制剂提供结构依据。
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**注意**：以上文献为模拟示例，实际引用请通过学术数据库(如PubMed、Web of Science)核实具体内容。建议以“recombinant human CMIP”“CMIP protein function”为关键词检索最新文献。
**Background of Recombinant Human CMIP Protein**
The recombinant human CMIP (C-Maf-inducing protein) is a genetically engineered version of the CMIP protein, produced using heterologous expression systems like *E. coli*, yeast, or mammalian cell lines. CMIP, encoded by the *CMIP* gene located on chromosome 16q23. is a cytosolic protein implicated in cellular signaling, immune regulation, and cytoskeletal dynamics. Though its precise biological roles remain under investigation, CMIP interacts with key signaling molecules, including components of the Wnt pathway, and may influence T-cell activation, podocyte function in the kidney, and neuronal development. Dysregulation of CMIP has been linked to diseases such as focal segmental glomerulosclerosis (FSGS), certain cancers, and neurodevelopmental disorders.
Recombinant CMIP enables *in vitro* and *in vivo* studies to dissect its molecular mechanisms, including binding partners, post-translational modifications, and pathway interactions. Its production facilitates research into therapeutic targeting, such as modulating immune responses or addressing CMIP-associated pathologies. Challenges in producing functional recombinant CMIP include optimizing solubility, stability, and post-translational modifications, often requiring tailored expression systems. Current applications span basic research, disease modeling, and drug discovery, positioning it as a potential biomarker or therapeutic candidate in precision medicine approaches. Further studies are needed to clarify its multifunctional roles and translational potential.
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