| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CMAH |
| Uniprot No | Q9Y471 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 385-485aa |
| 氨基酸序列 | GYDYLVDFLDLSFPKERPQREHPYEEIHSRVDVIRHVVKNGLLWDELYIGFQTRLQRDPDIYHHLFWNHFQIKLPLTPPNWKSFLMCCEQNGPAILQECKT |
| 分子量 | 36.85 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CMAH蛋白的3篇代表性文献的简要总结(注：文献信息为示例性质，具体内容需查询实际数据库验证)：
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1. **文献名称**: "Expression and characterization of recombinant human CMAH enzyme in mammalian cells"
**作者**: Yamamoto A. et al.
**摘要**: 研究通过哺乳动物表达系统成功制备了具有生物活性的重组人CMAH蛋白，并验证其在唾液酸代谢中的催化功能，证实其可用于体外研究神经氨酸(Neu5Gc)的合成机制。
2. **文献名称**: "Structural insights into the catalytic mechanism of human CMAH through recombinant protein crystallography"
**作者**: Chen L. et al.
**摘要**: 通过重组表达纯化获得人源CMAH蛋白，解析其晶体结构并揭示其底物结合域和羟化反应的关键氨基酸残基，为靶向药物设计提供结构基础。
3. **文献名称**: "CMAH knockout human cell lines generated via CRISPR-Cas9 reveal the role of CMAH in cellular glycosylation"
**作者**: Varki N. et al.
**摘要**: 利用重组CMAH蛋白作为对照工具，研究CMAH基因敲除对细胞表面糖基化的影响，发现其缺失导致Neu5Gc合成完全终止，印证了CMAH在唾液酸修饰中的核心作用。
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**备注**：以上文献为领域内典型研究方向(表达、结构、功能研究)的假设性示例，实际文献需通过PubMed、Web of Science等平台以关键词“recombinant human CMAH”或“CMAH protein expression”检索获取。如需具体文献，建议结合研究场景进一步筛选。
The cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) enzyme catalyzes the synthesis of CMP-N-glycolylneuraminic acid (Neu5Gc) from CMP-N-acetylneuraminic acid (Neu5Ac), a critical step in sialic acid metabolism. In humans, an evolutionary loss-of-function mutation in the CMAH gene rendered the enzyme inactive, making Neu5Gc absent in human tissues. However, Neu5Gc is prevalent in other mammals and is incorporated into human cells through dietary sources (e.g., red meat), potentially contributing to inflammation and immune responses due to its immunogenicity. Recombinant human CMAH protein, produced via genetic engineering in heterologous systems like bacteria or mammalian cells, serves as a key tool to study sialic acid biology, species-specific pathogen interactions, and Neu5Gc-related pathologies. Its applications span glycobiology research, xenotransplantation studies (e.g., addressing immune rejection of animal-derived organs), and cancer biology, as Neu5Gc expression has been implicated in tumor progression and immune evasion. Additionally, recombinant CMAH enables the engineering of cell surfaces with Neu5Gc for immunological studies or biotherapeutic development. Understanding CMAH's structure and function also aids in elucidating evolutionary adaptations and developing strategies to modulate sialic acid-mediated cellular processes.
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