| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLYBL |
| Uniprot No | Q8N0X4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MALRLLRRAARGAAAAALLRLKASLAADIPRLGYSSSSHHKYIPRRAVLYVPGNDEKKIKKIPSLNVDCAVLDCEDGVAANKKNEARLRIVKTLEDIDLGPTEKCVRVNSVSSGLAEEDLETLLQSRVLPSSLMLPKVESPEEIQWFADKFSFHLKGRKLEQPMNLIPFVETAMGLLNFKAVCEETLKVGPQVGLFLDAVVFGGEDFRASIGATSSKETLDILYARQKIVVIAKAFGLQAVDLVYIDFRDGAGLLRQSREGAAMGFTGKQVIHPNQIAVVQEQFSPSPEKIKWAEELIAAFKEHQQLGKGAFTFQGSMIDMPLLKQAQNTVTLATSIKEK |
| 分子量 | 63.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CLYBL蛋白的3-4篇文献示例，基于学术研究方向的代表性总结(注：部分文献信息为模拟生成，供参考)：
1. **《Clybl knockout mouse model reveals metabolic adaptations linking citrate and glutathione pathways》**
- **作者**: Haber, L. et al. (2015)
- **摘要**: 通过构建Clybl基因敲除小鼠，研究CLYBL蛋白缺失对柠檬酸代谢通路的影响，揭示其通过调节柠檬酸裂解酶活性在细胞抗氧化(谷胱甘肽合成)中的潜在作用。
2. **《Expression and enzymatic characterization of recombinant human CLYBL in E. coli》**
- **作者**: Al-Khalli, J. et al. (2017)
- **摘要**: 报道了使用大肠杆菌表达系统成功制备重组人CLYBL蛋白的优化方法，并通过体外酶活实验证实其催化柠檬酸生成乙酰辅酶A的能力，为功能研究提供工具。
3. **《Crystal structure of human CLYBL reveals a novel dimeric assembly in the citrate lyase family》**
- **作者**: Smith, T.L. & Johnson, R.K. (2020)
- **摘要**: 首次解析了重组人CLYBL蛋白的晶体结构，发现其独特的二聚体构象和活性位点特征，揭示了与其他物种同源蛋白的进化差异。
4. **《CLYBL suppresses tumorigenesis by modulating mitochondrial citrate flux in prostate cancer》**
- **作者**: Zhang, Y. et al. (2022)
- **摘要**: 利用重组CLYBL蛋白进行功能回补实验，证明其通过抑制线粒体柠檬酸转运影响癌细胞的脂质合成，提出其作为抑癌因子的机制。
**注**：以上文献为领域方向模拟示例，具体研究请以真实发表的论文为准。建议通过PubMed或Web of Science以“CLYBL protein recombinant”或“human CLYBL”为关键词检索最新文献。
CLYBL (Citrate Lyase Beta-Like) is a human gene encoding a mitochondrial enzyme implicated in cellular metabolism, particularly in citrate and acetyl-CoA regulation. The protein is thought to function as a citryl-CoA lyase, catalyzing the cleavage of citryl-CoA into acetyl-CoA and oxaloacetate—a critical step in intermediary metabolism. CLYBL's activity influences metabolic flux, energy balance, and lipid synthesis, with potential links to neurological disorders, cancer, and metabolic syndromes. Reduced CLYBL expression has been associated with altered mitochondrial function and metabolic inflexibility.
Recombinant human CLYBL protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional and structural studies. Its production allows researchers to probe enzymatic mechanisms, substrate specificity, and interactions with metabolic pathways. Recent studies highlight its role in modulating intracellular acetyl-CoA levels, which are vital for histone acetylation, gene regulation, and cellular differentiation. Additionally, population genetics research reveals that CLYBL polymorphisms may affect vitamin B12 metabolism, suggesting broader physiological implications.
Current applications include biochemical assays, drug discovery targeting metabolic diseases, and elucidating CLYBL's involvement in rare genetic disorders. However, challenges remain in fully characterizing its in vivo regulation and tissue-specific roles. Recombinant CLYBL serves as a valuable tool to decode its contributions to metabolic health and disease.
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