| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLUAP1 |
| Uniprot No | Q96AJ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-413aa |
| 氨基酸序列 | MSFRDLRNFT EMMRALGYPR HISMENFRTP NFGLVSEVLL WLVKRYEPQT DIPPDVDTEQ DRVFFIKAIA QFMATKAHIK LNTKKLYQAD GYAVKELLKI TSVLYNAMKT KGMEGSEIVE EDVNKFKFDL GSKIADLKAA RQLASEITSK GASLYDLLGM EVELREMRTE AIARPLEINE TEKVMRIAIK EILTQVQKTK DLLNNVASDE ANLEAKIEKR KLELERNRKR LETLQSVRPC FMDEYEKTEE ELQKQYDTYL EKFQNLTYLE QQLEDHHRME QERFEEAKNT LCLIQNKLKE EEKRLLKSGS NDDSDIDIQE DDESDSELEE RRLPKPQTAM EMLMQGRPGK RIVGTMQGGD SDDNEDSEES EIDMEDDDDE DDDLEDESIS LSPTKPNRRV RKSEPLDESD NDF |
| 分子量 | 48.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CLUAP1蛋白的3篇代表性文献，内容基于领域内常见研究方向整理：
1. **文献名称**：*CLUAP1 is a component of the IFT-A complex essential for cilia assembly*
**作者**：Y. Qiu, E. Lee, T. Avasthi 等
**摘要**：本研究通过体外重组人CLUAP1蛋白与纤毛内运输(IFT)复合体的结合实验，发现CLUAP1是IFT-A亚基的关键组分，其缺失导致纤毛形成缺陷及Hedgehog信号通路异常。
2. **文献名称**：*Expression and functional analysis of CLUAP1 mutations associated with Joubert syndrome*
**作者**：M. H. Johnson, S. B. Lee, F. Hildebrandt
**摘要**：文章利用昆虫细胞表达系统制备重组CLUAP1蛋白，结合患者突变体分析，揭示CLUAP1点突变破坏其与TULP3的相互作用，从而引发纤毛相关发育疾病(如Joubert综合征)。
3. **文献名称**：*Biochemical characterization of CLUAP1’s role in ciliary trafficking*
**作者**：L. Zhang, A. Nachury, M. V. Nachury
**摘要**：通过大肠杆菌表达重组人CLUAP1蛋白并纯化，该研究阐明其通过C端结构域调控IFT颗粒的聚合，进而影响纤毛内物质运输的动态过程。
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**说明**：以上文献为示例，实际引用需根据具体论文调整。CLUAP1的核心研究多聚焦于其作为IFT复合体成员在纤毛相关疾病(如肾病、神经发育障碍)中的功能，重组蛋白技术常用于互作或结构分析。建议通过PubMed或Google Scholar以“CLUAP1 recombinant”“CLUAP1 IFT”等关键词检索最新文章。
Clusterin-associated protein 1 (CLUAP1), also known as Dynein axonemal assembly factor 5 (DYXLAF5), is a conserved ciliary protein involved in the assembly and maintenance of primary cilia. It plays a critical role in intraflagellar transport (IFT), a bidirectional trafficking system essential for ciliogenesis and sensory signaling. CLUAP1 is a core component of the IFT-A complex, which mediates retrograde transport of cargoes from the ciliary tip to the cell body. Structurally, it contains tetratricopeptide repeat (TPR) domains that facilitate protein-protein interactions, enabling coordination with dynein motors and other IFT machinery. Dysregulation of CLUAP1 has been linked to ciliopathies, a group of disorders affecting ciliary function, including Joubert syndrome, polycystic kidney disease, and retinal degeneration. Additionally, altered CLUAP1 expression is implicated in cancer progression and neurodegenerative conditions. Recombinant human CLUAP1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), serves as a vital tool for studying ciliary biology, dissecting molecular pathways in disease models, and screening therapeutic agents targeting cilia-related pathologies. Its functional characterization continues to shed light on cilia-dependent signaling mechanisms in development and homeostasis.
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