| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLK2 |
| Uniprot No | Q86UE8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-772aa |
| 氨基酸序列 | MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETPSSGNTELKDTAPALGAHSLLRLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN |
| 预测分子量 | 87kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TLK2重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括列出：
1. **文献名称**：*"Expression and Characterization of Recombinant Human TLK2 Kinase"*
**作者**：Smith A, et al.
**摘要**：该研究报道了人源TLK2激酶的重组表达及纯化方法，利用大肠杆菌系统成功获得高活性蛋白，并分析了其体外磷酸化底物的特性，为后续激酶功能研究提供了工具。
2. **文献名称**：*"TLK2 Recombinant Protein Enhances DNA Repair in Cellular Models"*
**作者**：Chen L, Wang Y.
**摘要**：研究通过昆虫细胞系统表达重组TLK2蛋白，发现其能促进DNA损伤修复过程，并揭示了TLK2与BRCA1蛋白的相互作用机制，提示其在癌症治疗中的潜在应用。
3. **文献名称**：*"Structural Insights into TLK2 Activation by Recombinant Co-Expression with Chaperones"*
**作者**：Gomez-Ruiz S, et al.
**摘要**：利用哺乳动物细胞共表达系统，结合分子伴侣蛋白优化TLK2重组蛋白的活性构象，通过晶体结构解析阐明了其自抑制状态的调控机制。
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注：以上文献信息为示例性内容，实际文献需根据具体研究通过学术数据库检索。建议使用关键词“TLK2 recombinant protein”或“TLK2 kinase purification”在PubMed或Google Scholar中查找最新研究。
**Background of TLK2 Recombinant Protein**
TLK2 (Tousled-like kinase 2) is a serine/threonine kinase belonging to the Tousled-like kinase family, which shares structural and functional homology with TLK1. It plays critical roles in regulating chromatin assembly, DNA repair, and cell cycle progression. TLK2 is involved in phosphorylating key substrates such as histone chaperones ASF1A/B, which are essential for histone deposition during DNA replication and repair. This kinase also interacts with components of the DNA damage response (DDR) pathway, suggesting its role in maintaining genomic stability under stress conditions.
Recombinant TLK2 protein is engineered through molecular cloning, typically expressed in bacterial or mammalian systems to ensure proper post-translational modifications and functional activity. The recombinant form often includes affinity tags (e.g., GST, His-tag) for purification and detection. Researchers utilize TLK2 recombinant protein to study its enzymatic activity, substrate interactions, and regulatory mechanisms in vitro. Its dysregulation has been implicated in diseases, including cancer, where TLK2 overexpression correlates with tumor progression and poor prognosis.
Current studies focus on TLK2's potential as a therapeutic target, particularly in cancers with defective DDR pathways. Inhibitors targeting TLK2 are under investigation to disrupt cancer cell proliferation and sensitize tumors to chemotherapy or radiation. Additionally, TLK2 recombinant protein aids in structural studies to map its kinase domain and regulatory regions, providing insights for drug design.
Overall, TLK2 recombinant protein serves as a vital tool for deciphering its biological functions and exploring translational applications in oncology and molecular biology.
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