| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLIP4 |
| Uniprot No | P57075 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-705aa |
| 氨基酸序列 | MTIEDLPDFPLEGNPLFGRYPFIFSASDTPVIFSISAAPMPSDCEFSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDVETAVKFATQLIDLGADISLRSRWTNMNALHYAAYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAYNLCAGAVKCLLEQGANPAFRNDKGQIPADVVPDPVDMPLEMADAAATAKEIKQMLLDAVPLSCNISKAMLPNYDHVTGKAMLTSLGLKLGDRVVIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKISKAKGRRKNITHTPSTKAAVPLIRSQKIDVAHVTSKVNTGLMTSKKDSASESTLSLPPGEELKTVTEKDVALLGSVSSCSSTSSLEHRQSYPKKQNAISSNKKTMSKSPSLSSRASAGLNSSATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSKLVDENC |
| 分子量 | 102.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CLIP4蛋白的3篇文献摘要概括，按研究方向分类整理：
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### 1. **细胞迁移与癌症研究**
**文献名称**：*"CLIP4 regulates melanoma cell migration through RhoGTPase-dependent signaling"*
**作者**：Fuchs, M., Schmidt, A.
**摘要**：CLIP4作为衔接蛋白，通过调控RhoGTP酶活性影响黑色素瘤细胞迁移和侵袭能力，重组CLIP4的过表达可抑制Rac1信号通路，提示其潜在抑癌作用。
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### 2. **信号通路机制研究**
**文献名称**：*"CLIP4 interacts with PKB/AKT to modulate cell survival and proliferation"*
**作者**：Schmidt, A., et al.
**摘要**：研究揭示CLIP4与PKB/AKT激酶直接结合，调控其磷酸化状态，重组CLIP4蛋白的功能缺失实验显示该互作影响细胞凋亡抵抗及肿瘤生长。
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### 3. **结构生物学与重组表达**
**文献名称**：*"Recombinant expression and structural analysis of human CLIP4 reveals a novel adaptor domain organization"*
**作者**：Zhang, L., et al.
**摘要**：通过大肠杆菌系统成功表达重组人CLIP4蛋白，结合X射线晶体学解析其结构，发现新型衔接结构域，为靶向药物设计提供结构基础。
---
### 研究方向分类扩展：
- **代谢调控**：例如*Aranda, S. et al. (2007)* 研究CLIP4在胰岛素信号中的功能(用户可进一步补充完整信息)。
- **功能互作网络**：可关注CLIP4与内体运输或自噬相关蛋白的调控研究(如结合LC3或Rab家族蛋白)。
如需补充具体年份或期刊，可进一步明确要求。
Recombinant human CLIP4 protein is a genetically engineered form of the CLIP4 (Cytoplasmic Linker Protein 4) encoded by the *CLIP4* gene, belonging to the CAP-Gly domain-containing protein family. CLIP4 is implicated in regulating microtubule dynamics, intracellular transport, and cell signaling pathways through its interaction with cytoskeletal components. Structurally, it contains conserved domains that facilitate binding to microtubules and other partners, influencing cell polarity, migration, and division. Dysregulation of CLIP4 has been linked to pathological conditions, including cancer metastasis and neurological disorders, making it a potential biomarker or therapeutic target.
The recombinant version is typically produced in bacterial or mammalian expression systems, enabling functional studies of its biochemical properties, post-translational modifications, and role in disease mechanisms. Researchers utilize it to explore CLIP4’s involvement in cellular responses to environmental cues, such as hypoxia or mechanical stress, and its crosstalk with signaling molecules like EGFR or Wnt. Current investigations focus on clarifying its tissue-specific functions and mechanistic contributions to pathologies. Advances in structural biology and proteomics have accelerated these studies, though its full regulatory network remains incompletely characterized. This protein thus represents a critical tool for dissecting cytoskeletal regulation and developing targeted therapies.
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