| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLIP2 |
| Uniprot No | Q9UDT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1046aa |
| 氨基酸序列 | MQKPSGLKPPGRGGKHSSPMGRTSTGSASSSAAVAASSKEGSPLHKQSSGPSSSPAAAAAPEKPGPKAAEVGDDFLGDFVVGERVWVNGVKPGVVQYLGETQFAPGQWAGVVLDDPVGKNDGAVGGVRYFECPALQGIFTRPSKLTRQPTAEGSGSDAHSVESLTAQNLSLHSGTATPPLTSRVIPLRESVLNSSVKTGNESGSNLSDSGSVKRGEKDLRLGDRVLVGGTKTGVVRYVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCPPKFGLFAPIHKVIRIGFPSTSPAKAKKTKRMAMGVSALTHSPSSSSISSVSSVASSVGGRPSRSGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHICEVEKEIALLKAQHEQYVAEAEEKLQRARLLVESVRKEKVDLSNQLEEERRKVEDLQFRVEEESITKGDLETQTQLEHARIGELEQSLLLEKAQAERLLRELADNRLTTVAEKSRVLQLEEELTLRRGEIEELQQCLLHSGPPPPDHPDAAEILRLRERLLSASKEHQRESGVLRDKYEKALKAYQAEVDKLRAANEKYAQEVAGLKDKVQQATSENMGLMDNWKSKLDSLASDHQKSLEDLKATLNSGPGAQQKEIGELKAVMEGIKMEHQLELGNLQAKHDLETAMHVKEKEALREKLQEAQEELAGLQRHWRAQLEVQASQHRLELQEAQDQRRDAELRVHELEKLDVEYRGQAQAIEFLKEQISLAEKKMLDYERLQRAEAQGKQEVESLREKLLVAENRLQAVEALCSSQHTHMIESNDISEETIRTKETVEGLQDKLNKRDKEVTALTSQTEMLRAQVSALESKCKSGEKKVDALLKEKRRLEAELETVSRKTHDASGQLVLISQELLRKERSLNELRVLLLEANRHSPGPERDLSREVHKAEWRIKEQKLKDDIRGLREKLTGLDKEKSLSDQRRYSLIDPSSAPELLRLQHQLMSTEDALRDALDQAQQVEKLMEAMRSCPDKAQTIGNSGSANGIHQQDKAQKQEDKH |
| 分子量 | 142.01 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CLIP2蛋白的3篇参考文献示例(注：内容基于研究领域常见方向推测，实际文献需核实)：
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1. **文献名称**：*Recombinant Human CLIP2 Expression and Its Role in Neurodevelopmental Disorders*
**作者**：Chen L, et al.
**摘要**：本研究通过昆虫细胞系统成功表达并纯化了重组人CLIP2蛋白，发现其与微管结合蛋白相互作用异常可能导致神经发育障碍，为相关疾病的分子机制提供了新见解。
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2. **文献名称**：*CLIP2 Mutations in Thyroid Cancer: Functional Analysis Using Recombinant Protein Models*
**作者**：Park S, et al.
**摘要**：通过大肠杆菌表达重组人CLIP2蛋白，揭示了其突变体通过干扰EGFR信号通路促进甲状腺癌转移，为靶向治疗提供了潜在靶点。
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3. **文献名称**：*Structural Characterization of Recombinant Human CLIP2 and Its Interaction with Microtubules*
**作者**：Garcia-Ruiz I, et al.
**摘要**：利用X射线晶体学解析了重组人CLIP2蛋白的结构，并证明其CAP-Gly结构域对微管动态调节至关重要，为癌症细胞骨架研究奠定基础。
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**备注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等数据库检索真实文献。CLIP2相关研究多聚焦于其在细胞骨架调控、癌症及神经系统疾病中的功能。
Recombinant human CLIP2 (CAP-Gly domain-containing linker protein 2) is a engineered protein derived from the CLIP2 gene, which encodes a cytoskeletal linker molecule involved in intracellular transport and microtubule dynamics. CLIP2 belongs to the CLIP family of proteins that interact with microtubule plus-end tracking proteins (+TIPs), facilitating connections between microtubules and cellular organelles or the actin cytoskeleton. It contains a conserved N-terminal CAP-Gly domain critical for binding to α-tubulin and other partners, enabling its role in regulating vesicle trafficking, cell polarization, and mitotic spindle organization.
CLIP2 has garnered research interest due to its potential involvement in neurological disorders and cancer. Studies suggest it may influence neuronal development by modulating microtubule stability in axons and dendrites. Dysregulation of CLIP2 has been observed in certain gliomas and autoimmune conditions. The recombinant form, typically produced in E. coli or mammalian expression systems, allows precise study of its biochemical interactions, structural motifs, and post-translational modifications. Its applications span from elucidating cellular transport mechanisms to screening therapeutic compounds targeting microtubule-associated pathways. Recent work also explores CLIP2's role in immune cell signaling, highlighting its multifaceted functions beyond structural scaffolding.
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