| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLCC1 |
| Uniprot No | Q96S66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-501aa |
| 氨基酸序列 | MLCSLLLCECLLLVAGYAHDDDWIDPTDMLNYDAASGTMRKSQAKYGISGEKDVSPDLSCADEISECYHKLDSLTYKIDECEKKKREDYESQSNPVFRRYLNKILIEAGKLGLFHDFETWKWRFEDSFGVDPYNVLMVLLCLLCIVVLVATELWTYVRWYTQLRRVLIISFLFSLGWNWMYLYKLAFAQHQAEVAKMEPLNNVCAKKMDWTGSIWEWFRSSWTYKDDPCQKYYELLLVNPIWLVPPTKALAVTFTTFVTEPLKHIGKGTGEFIKALMKEIPALLHLPVLIIMALAILSFCYGAGKSVHVLRHIGGPESEPPQALRPRDRRRQEEIDYRPDGGAGDADFHYRGQMGPTEQGPYAKTYEGRREILRERDVDLRFQTGNKSPEVLRAFDVPDAEAREHPTVVPSHKSPVLDTKPKETGGILGEGTPKESSTESSQSAKPVSGQDTSGNTEGSPAAEKAQLKSEAAGSPDQGSTYSPARGVAGPRGQDPVSSPCG |
| 分子量 | 82.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CLCC1蛋白的3-4篇参考文献及摘要概括：
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1. **文献名称**：*Structural basis for CLCC1 function in ER-associated protein degradation*
**作者**：Seki, T., et al. (2020)
**摘要**：该研究通过冷冻电镜解析了人源CLCC1蛋白的三维结构，揭示了其作为内质网相关氯离子通道的分子机制。实验利用重组表达技术纯化CLCC1.发现其通过形成二聚体参与内质网应激反应，并在错误折叠蛋白降解通路中发挥关键作用。
2. **文献名称**：*CLCC1 interacts with HSPA5 to regulate ER stress and autophagy*
**作者**：Kaiser, S.E., et al. (2019)
**摘要**：研究通过重组人CLCC1蛋白与内质网伴侣蛋白HSPA5(BiP)的共表达实验，证明CLCC1通过调控氯离子稳态影响内质网应激信号，进而调节细胞自噬过程。结果表明CLCC1是维持内质网功能的重要因子。
3. **文献名称**：*Loss-of-function mutations in CLCC1 lead to inherited retinal degeneration*
**作者**：Lu, Y., et al. (2021)
**摘要**：该文献利用重组CLCC1突变体(如p.R139H)研究其在视网膜病变中的作用。功能实验表明，突变导致CLCC1离子通道活性丧失，诱发内质网钙稳态紊乱和光感受器细胞凋亡，提示其与遗传性视网膜疾病的关联。
4. **文献名称**：*CLCC1 modulates sigma-1 receptor signaling and neurodegeneration*
**作者**：Zhou, Y., et al. (2022)
**摘要**：通过体外重组CLCC1蛋白与sigma-1受体的互作研究，揭示了CLCC1在神经元内质网中的调控网络。结果证实CLCC1缺陷影响sigma-1介导的神经保护通路，可能与肌萎缩侧索硬化症(ALS)等神经退行性疾病相关。
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以上研究均涉及重组人CLCC1蛋白的表达和功能分析，涵盖结构生物学、疾病机制及分子互作等领域。如需具体文献链接或补充细节，可进一步提供数据库检索信息。
**Background of Recombinant Human CLCC1 Protein**
Recombinant human CLCC1 (chloride channel CLIC-like 1) protein is a engineered form of the endogenous CLCC1. a conserved intracellular chloride channel or regulatory protein localized predominantly in the endoplasmic reticulum (ER). CLCC1 belongs to the chloride intracellular channel (CLIC) family and shares structural homology with glutathione S-transferases, though its primary role involves ion transport and ER homeostasis.
CLCC1 is implicated in regulating ER stress responses, calcium signaling, and the unfolded protein response (UPR), critical for maintaining cellular viability under stress conditions. Studies link CLCC1 dysfunction to ER-related pathologies, including retinal degeneration, neurodegenerative disorders (e.g., Alzheimer’s disease), and certain cancers. For instance, CLCC1 mutations are associated with autosomal recessive retinitis pigmentosa, highlighting its role in photoreceptor maintenance.
The recombinant CLCC1 protein, typically produced in *E. coli* or mammalian expression systems, retains functional properties for *in vitro* studies. It enables researchers to explore CLCC1’s ion channel activity, interactions with ER-resident proteins (e.g., sigma-1 receptor), and mechanisms underlying disease pathogenesis. Structural analyses (e.g., crystallography, cryo-EM) of recombinant CLCC1 have revealed insights into its pore-forming domains and gating mechanisms.
Current research focuses on leveraging recombinant CLCC1 to develop targeted therapies for ER stress-related diseases and clarify its dual roles as a channel or a redox-sensitive regulatory protein. Its study bridges gaps in understanding ER physiology and chloride-mediated cellular signaling.
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