| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLCA2 |
| Uniprot No | Q9UQC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-943aa |
| 氨基酸序列 | MTQRSIAGPICNLKFVTLLVALSSELPFLGAGVQLQDNGYNGLLIAINPQVPENQNLISNIKEMITEASFYLFNATKRRVFFRNIKILIPATWKANNNSKIKQESYEKANVIVTDWYGAHGDDPYTLQYRGCGKEGKYIHFTPNFLLNDNLTAGYGSRGRVFVHEWAHLRWGVFDEYNNDKPFYINGQNQIKVTRCSSDITGIFVCEKGPCPQENCIISKLFKEGCTFIYNSTQNATASIMFMQSLSSVVEFCNASTHNQEAPNLQNQMCSLRSAWDVITDSADFHHSFPMNGTELPPPPTFSLVQAGDKVVCLVLDVSSKMAEADRLLQLQQAAEFYLMQIVEIHTFVGIASFDSKGEIRAQLHQINSNDDRKLLVSYLPTTVSAKTDISICSGLKKGFEVVEKLNGKAYGSVMILVTSGDDKLLGNCLPTVLSSGSTIHSIALGSSAAPNLEELSRLTGGLKFFVPDISNSNSMIDAFSRISSGTGDIFQQHIQLESTGENVKPHHQLKNTVTVDNTVGNDTMFLVTWQASGPPEIILFDPDGRKYYTNNFITNLTFRTASLWIPGTAKPGHWTYTLNNTHHSLQALKVTVTSRASNSAVPPATVEAFVERDSLHFPHPVMIYANVKQGFYPILNATVTATVEPETGDPVTLRLLDDGAGADVIKNDGIYSRYFFSFAANGRYSLKVHVNHSPSISTPAHSIPGSHAMYVPGYTANGNIQMNAPRKSVGRNEEERKWGFSRVSSGGSFSVLGVPAGPHPDVFPPCKIIDLEAVKVEEELTLSWTAPGEDFDQGQATSYEIRMSKSLQNIQDDFNNAILVNTSKRNPQQAGIREIFTFSPQISTNGPEHQPNGETHESHRIYVAIRAMDRNSLQSAVSNIAQAPLFIPPNSDPVPARDYLILKGVLTAMGLIGIICLIIVVTHHTLSRKKRADKKENGTKLL |
| 分子量 | 103.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条与重组人CLCA2蛋白相关的虚构参考文献示例(仅供格式参考)：
1. **文献名称**：重组人CLCA2蛋白在乳腺癌细胞迁移抑制中的作用
**作者**：Smith J, et al.
**摘要**：本研究通过在大肠杆菌中表达纯化重组人CLCA2蛋白，发现其可通过调控钙依赖性信号通路抑制MDA-MB-231乳腺癌细胞的迁移能力，提示其潜在肿瘤抑制作用。
2. **文献名称**：CLCA2蛋白的重组表达及其对上皮屏障功能的影响
**作者**：Wang L, et al.
**摘要**：利用HEK293细胞系统重组表达人CLCA2蛋白，发现其通过增强紧密连接蛋白ZO-1的表达，显著改善体外模型中的上皮屏障完整性，为治疗皮肤损伤提供新思路。
3. **文献名称**：CLCA2重组蛋白在氧化应激诱导的细胞凋亡中的保护机制
**作者**：Kim S, et al.
**摘要**：通过体外实验证明，重组人CLCA2蛋白可通过激活Nrf2/ARE抗氧化通路，减少ROS累积，从而降低紫外线诱导的人角质形成细胞凋亡率。
The chloride channel accessory 2 (CLCA2) protein belongs to the CLCA family, initially identified as calcium-activated chloride channels. However, emerging evidence suggests CLCA members, including CLCA2. may function as cell adhesion regulators rather than ion channels. Human CLCA2 is highly expressed in epithelial tissues, particularly skin, mammary glands, and hair follicles, where it contributes to cell-matrix interactions, differentiation, and barrier formation. Structurally, it contains a conserved N-terminal metalloprotease-like domain and a C-terminal von Willebrand factor domain, facilitating extracellular matrix binding.
CLCA2 is implicated in cancer progression, acting as a tumor suppressor in breast cancer by inhibiting epithelial-mesenchymal transition (EMT) and metastasis. Its downregulation correlates with poor prognosis. Recombinant human CLCA2 protein, typically produced in mammalian or bacterial expression systems, enables functional studies exploring its role in cellular adhesion, signaling pathways (e.g., integrin-mediated), and cancer biology. It serves as a critical tool for in vitro assays, therapeutic target validation, and potential biomarker development. Current research focuses on harnessing recombinant CLCA2 for regenerative medicine and anticancer strategies, leveraging its ECM-modulating properties.
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