| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKMT1B |
| Uniprot No | P12532 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 40-417aa |
| 氨基酸序列 | ASERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFADLFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPTPVIHTKH |
| 分子量 | 47.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CKMT1B蛋白的参考文献示例(注：部分为虚构案例，实际文献需根据具体数据库检索)：
1. **文献名称**：*Expression and Characterization of Recombinant Human CKMT1B in Escherichia coli*
**作者**：Zhang L, et al.
**摘要**：该研究利用大肠杆菌表达系统成功表达并纯化重组人CKMT1B蛋白，通过酶活性实验证实其具有线粒体肌酸激酶的典型功能，并分析其在不同组织中的能量代谢调控作用。
2. **文献名称**：*Structural Insights into CKMT1B in Mitochondrial Energy Homeostasis*
**作者**：Wang H, et al.
**摘要**：通过X射线晶体学解析CKMT1B的三维结构，揭示其底物结合位点及ATP/ADP调控机制，为理解其在心肌细胞能量缓冲中的作用提供结构基础。
3. **文献名称**：*CKMT1B Overexpression Protects Against Cardiac Ischemia-Reperfusion Injury*
**作者**：Chen Y, et al.
**摘要**：在心肌细胞中过表达重组CKMT1B蛋白，发现其通过维持线粒体磷酸肌酸水平，显著减少缺血再灌注损伤中的细胞凋亡和氧化应激。
4. **文献名称**：*Role of CKMT1B in Tumor Cell Bioenergetics*
**作者**：Gupta S, et al.
**摘要**：研究重组CKMT1B在癌细胞中的功能，表明其通过促进线粒体能量转移，支持高能量需求肿瘤细胞的存活和增殖。
(注：以上文献标题与摘要为示例，实际引用时请检索PubMed、Google Scholar等平台获取真实文献。)
**Background of Recombinant Human CKMT1B Protein**
The human creatine kinase mitochondrial 1B (CKMT1B) protein, a member of the creatine kinase (CK) family, is a key enzyme involved in cellular energy metabolism. Specifically, it localizes to mitochondria and plays a critical role in the phosphocreatine shuttle system. This system facilitates ATP regeneration by catalyzing the reversible transfer of a high-energy phosphate group from ATP to creatine, generating phosphocreatine (PCr), which serves as an energy buffer in tissues with high metabolic demands, such as cardiac muscle, skeletal muscle, and brain.
CKMT1B is encoded by the *CKMT1* gene and represents a tissue-specific isoform, predominantly expressed in the heart and testes. Structurally, it contains conserved catalytic domains essential for its enzymatic activity and mitochondrial targeting sequences that direct its localization. Dysregulation of CKMT1B has been implicated in mitochondrial dysfunction, cardiomyopathy, and neurodegenerative disorders, highlighting its physiological significance.
Recombinant human CKMT1B protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling high-purity production for functional studies. It serves as a vital tool for investigating CKMT1B's enzymatic properties, interactions with mitochondrial substrates, and roles in cellular bioenergetics. Additionally, recombinant CKMT1B is used to explore therapeutic strategies targeting energy metabolism defects and to develop diagnostic biomarkers for diseases associated with mitochondrial impairment. Its study contributes to advancing knowledge in metabolic disorders, cardiovascular research, and neurobiology.
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