| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CITED4 |
| Uniprot No | Q96RK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-184aa |
| 氨基酸序列 | MADHLMLAEG YRLVQRPPSA AAAHGPHALR TLPPYAGPGL DSGLRPRGAP LGPPPPRQPG ALAYGAFGPP SSFQPFPAVP PPAAGIAHLQ PVATPYPGRA AAPPNAPGGP PGPQPAPSAA APPPPAHALG GMDAELIDEE ALTSLELELG LHRVRELPEL FLGQSEFDCF SDLGSAPPAG SVSC |
| 分子量 | 18.5 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人CITED4蛋白相关的代表性文献摘要概览：
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1. **文献名称**: *CITED4 Protects against Myocardial Ischemia-Reperfusion Injury by Regulating Mitochondrial Function*
**作者**: Hu, X. et al. (2020)
**摘要**: 该研究通过在大肠杆菌中表达重组人CITED4蛋白，发现其在心肌细胞中通过激活PGC-1α/NRF2通路调控线粒体生物合成，改善小鼠心脏缺血再灌注损伤模型中的心肌细胞凋亡和氧化应激，证实了CITED4的心脏保护作用。
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2. **文献名称**: *Functional Characterization of CITED4 Interaction with HIF-1α in Hypoxia Signaling*
**作者**: Kim, S. et al. (2018)
**摘要**: 研究利用体外纯化的重组CITED4蛋白和HIF-1α蛋白，通过Co-IP和荧光共振能量转移(FRET)实验证明，CITED4直接结合HIF-1α的氧依赖降解域(ODDD)，抑制其泛素化降解，从而增强缺氧条件下的血管生成因子(如VEGF)表达。
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3. **文献名称**: *Generation and Application of Recombinant CITED4 for Studying Cardiac Regeneration in Zebrafish*
**作者**: Wang, L. et al. (2021)
**摘要**: 通过昆虫杆状病毒系统表达具有His标签的重组CITED4.纯化后将其显微注射到斑马鱼心脏损伤模型中，发现CITED4显著促进心肌细胞增殖和损伤修复，机制涉及调控Wnt/β-catenin信号通路的活性。
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4. **文献名称**: *CITED4 Promotes Stem Cell Differentiation via Chromatin Remodeling*
**作者**: Gupta, R. et al. (2019)
**摘要**: 利用重组CITED4蛋白处理小鼠胚胎干细胞，发现其通过招募组蛋白乙酰转移酶p300到特定基因启动子区(如Oct4和Nanog)，诱导染色质重塑和干细胞向心肌细胞分化，为再生医学提供了潜在靶点。
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说明：以上为假设性文献概括，实际文献需通过PubMed、Web of Science等数据库检索核实。建议使用关键词“recombinant CITED4”、“CITED4 protein function”等进行文献筛选。
CITED4 (CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl-terminal domain 4) is a transcriptional co-regulator belonging to the CITED family, which modulates gene expression by interacting with transcription factors and epigenetic modifiers. It functions as a hypoxia-inducible factor (HIF) pathway regulator by competing with HIF-1α for binding to the transcriptional coactivators CBP/p300. thereby fine-tuning cellular responses to low oxygen conditions. CITED4 is implicated in diverse biological processes, including angiogenesis, stress adaptation, and cell survival.
Recombinant human CITED4 protein is produced using genetic engineering techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This engineered protein retains functional domains required for interacting with partner molecules, enabling its use in mechanistic studies, protein-protein interaction assays, and functional characterization. Research highlights its cardioprotective roles, such as mitigating cardiac hypertrophy and promoting cardiomyocyte proliferation in injury models. The recombinant form has become crucial for exploring its therapeutic potential in ischemic diseases, tissue repair, and cancer biology. Its precise regulation of HIF and other pathways positions it as a potential target for modulating stress-responsive gene networks. Purified recombinant CITED4 is widely used in structural studies and drug discovery platforms.
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