| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHUK |
| Uniprot No | O15111 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-745aa |
| 氨基酸序列 | MERPPGLRPG AGGPWEMRER LGTGGFGNVC LYQHRELDLK IAIKSCRLEL STKNRERWCH EIQIMKKLNH ANVVKACDVP EELNILIHDV PLLAMEYCSG GDLRKLLNKP ENCCGLKESQ ILSLLSDIGS GIRYLHENKI IHRDLKPENI VLQDVGGKII HKIIDLGYAK DVDQGSLCTS FVGTLQYLAP ELFENKPYTA TVDYWSFGTM VFECIAGYRP FLHHLQPFTW HEKIKKKDPK CIFACEEMSG EVRFSSHLPQ PNSLCSLVVE PMENWLQLML NWDPQQRGGP VDLTLKQPRC FVLMDHILNL KIVHILNMTS AKIISFLLPP DESLHSLQSR IERETGINTG SQELLSETGI SLDPRKPASQ CVLDGVRGCD SYMVYLFDKS KTVYEGPFAS RSLSDCVNYI VQDSKIQLPI IQLRKVWAEA VHYVSGLKED YSRLFQGQRA AMLSLLRYNA NLTKMKNTLI SASQQLKAKL EFFHKSIQLD LERYSEQMTY GISSEKMLKA WKEMEEKAIH YAEVGVIGYL EDQIMSLHAE IMELQKSPYG RRQGDLMESL EQRAIDLYKQ LKHRPSDHSY SDSTEMVKII VHTVQSQDRV LKELFGHLSK LLGCKQKIID LLPKVEVALS NIKEADNTVM FMQGKRQKEI WHLLKIACTQ SSARSLVGSS LEGAVTPQTS AWLPPTSAEH DHSLSCVVTP QDGETSAQMI EENLNCLGHL STIIHEANEE QGNSMMNLDW SWLTE |
| 分子量 | 84.6 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CHUK蛋白的3篇参考文献，按研究领域和内容分类简要概括：
1. **文献名称**：*Reconstitution of the IκB Kinase Complex in Vitro Using Recombinant Human Proteins*
**作者**：Li, X.; Commane, M.; Nie, H.; Hua, X.; Chatterjee, D.; Stark, G.R.
**摘要**：该研究通过重组表达人源CHUK(IKK-α)、IKK-β和NEMO蛋白，在HEK293细胞中重建了功能性IKK复合物，证明其在体外能磷酸化IκBα并激活NF-κB通路，为激酶活性机制研究提供模型。
2. **文献名称**：*Structural Insight into the Interaction of CHUK with the SCFβ-TrCP Ubiquitin Ligase*
**作者**：Chen, J.; Wu, H.; Zhang, L.; et al.
**摘要**：通过X射线晶体学解析重组人CHUK与SCFβ-TrCP复合物的结构，揭示CHUK通过特定结构域介导泛素化降解的分子机制，阐明其在NF-κB信号转导中的调控作用。
3. **文献名称**：*Development of a High-Throughput Screening Assay for CHUK Kinase Inhibitors*
**作者**：Adams, R.; Thompson, C.; Davies, T.
**摘要**：开发基于重组人CHUK蛋白的激酶活性高通量筛选系统，用于发现NF-κB通路小分子抑制剂，验证其在抗炎和抗癌药物开发中的潜在应用价值。
**备注**：以上文献信息为示例性质，实际引用时需核实具体来源及真实性。若需精确文献，建议通过PubMed或Web of Science以“recombinant CHUK”、“IKK-alpha expression”等关键词检索近年研究。
The conserved helix-loop-helix ubiquitous kinase (CHUK), also known as IκB kinase-alpha (IKKα), is a serine/threonine kinase critical in regulating the NF-κB signaling pathway. Discovered in the 1990s, CHUK forms part of the IKK complex (with IKKβ and NEMO/IKKγ) that phosphorylates IκB inhibitors, triggering their ubiquitination and degradation. This releases NF-κB transcription factors to modulate genes involved in inflammation, immunity, apoptosis, and cell proliferation. Unlike IKKβ, CHUK also participates in NF-κB-independent processes, including epidermal differentiation and morphogenesis.
Recombinant human CHUK protein is engineered via heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its structure, enzymatic activity, and interactions. The purified protein retains kinase activity, enabling in vitro assays to explore substrate specificity, inhibitor screening, and pathway crosstalk. Its production typically involves cloning the CHUK cDNA into expression vectors, followed by affinity chromatography and functional validation.
Research applications span cancer, autoimmune diseases, and chronic inflammatory conditions, as dysregulated CHUK/IKKα is linked to tumor progression and immune disorders. Recombinant CHUK also aids drug discovery, particularly for targeted therapies against NF-κB-driven pathologies. Its role in non-canonical pathways further underscores its versatility as a research tool.
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