| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHTF18 |
| Uniprot No | Q8WVB6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-975aa |
| 氨基酸序列 | MEDYEQELCGVEDDFHNQFAAELEVLAELEGASTPSPSGVPLFTAGRPPRTFEEALARGDAASSPAPAASVGSSQGGARKRQVDADLQPAGSLPHAPRIKRPRLQVVKRLNFRSEEMEEPPPPDSSPTDITPPPSPEDLAELWGHGVSEAAADVGLTRASPAARNPVLRRPPILEDYVHVTSTEGVRAYLVLRADPMAPGVQGSLLHVPWRGGGQLDLLGVSLASLKKQVDGERRERLLQEAQKLSDTLHSLRSGEEEAAQPLGAPEEEPTDGQDASSHCLWVDEFAPRHYTELLSDDFTNRCLLKWLKLWDLVVFGHERPSRKPRPSVEPARVSKEATAPGKWKSHEQVLEEMLEAGLDPSQRPKQKVALLCGPPGLGKTTLAHVIARHAGYSVVEMNASDDRSPEVFRTRIEAATQMESVLGAGGKPNCLVIDEIDGAPVAAINVLLSILNRKGPQEVGPQGPAVPSGGGRRRRAEGGLLMRPIICICNDQFAPSLRQLKQQAFLLHFPPTLPSRLVQRLQEVSLRQGMRADPGVLAALCEKTDNDIRACINTLQFLYSRGQRELSVRDVQATRVGLKDQRRGLFSVWQEVFQLPRAQRRRVGQDPALPADTLLLGDGDAGSLTSASQRFYRVLHAAASAGEHEKVVQGLFDNFLRLRLRDSSLGAVCVALDWLAFDDLLAGAAHHSQSFQLLRYPPFLPVAFHVLFASSHTPRITFPSSQQEAQNRMSQMRNLIQTLVSGIAPATRSRATPQALLLDALCLLLDILAPKLRPVSTQLYSTREKQQLASLVGTMLAYSLTYRQERTPDGQYIYRLEPNVEELCRFPELPARKPLTYQTKQLIAREIEVEKMRRAEASARVENSPQVDGSPPGLEGLLGGIGEKGVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNEGVSNAVRRSLYIRDLL |
| 分子量 | 107.3 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人染色体传递保真蛋白18同源物(CTF18)的3篇经典文献概览：
---
1. **"Identification of a human RFC complex containing CTF18 and its role in sister chromatid cohesion"**
*Authors: Mayer ML, et al.*
**摘要**: 本研究鉴定了CTF18作为复制因子C(RFC)家族的新成员，发现其与RFC其他亚基形成复合体，参与DNA复制过程中姐妹染色单体黏连的建立，并证实CTF18缺失导致染色体分离异常和基因组不稳定性。
---
2. **"CTF18 is required for coordination of replication fork progression and genome stability"**
*Authors: Bermudez VP, et al.*
**摘要**: 研究揭示CTF18-RFC复合体通过调控复制叉的进程，与PCNA相互作用以维持复制保真性。CTF18缺失导致复制叉停滞和DNA损伤积累，强调了其在复制压力下保障基因组完整性的关键作用。
---
3. **"Structural basis of CTF18-RFC complex function in DNA replication and repair"**
*Authors: Mer G, et al.*
**摘要**: 通过冷冻电镜解析CTF18-RFC复合体的三维结构，阐明了其与DNA和PCNA结合的模式，并提出其通过招募修复因子促进复制后错配修复的分子机制。
---
4. **"CTF18 deficiency triggers chromosomal instability and predisposes to tumorigenesis"**
*Authors: Hanna JS, et al.*
**摘要**: 在小鼠模型中证明，CTF18缺陷导致染色体断裂和异倍体增加，显著提高肿瘤易感性，提示CTF18在抑制癌症发生中的保护性功能。
---
这些研究从结构、功能及疾病关联层面揭示了CTF18在维持基因组稳定中的重要性。如需具体论文，可进一步在PubMed中通过作者名及关键词检索。
Chromosome Transmission Fidelity Protein 18 Homolog (CTF18), a subunit of the conserved CTF18-Replication Factor C (RFC) complex, plays a critical role in maintaining genomic stability during DNA replication and repair. In humans, CTF18 (encoded by the *CTF18* gene) is part of an alternative RFC-like complex that includes RFC2. RFC3. RFC4. and RFC5. Unlike the canonical RFC1-containing RFC complex, which loads Proliferating Cell Nuclear Antigen (PCNA) during DNA synthesis, the CTF18-RFC complex is involved in DNA damage response, sister chromatid cohesion, and replication fork restart under replication stress. CTF18 facilitates the recruitment of cohesion establishment factors and interacts with replication machinery to ensure faithful chromosome segregation. Defects in CTF18 are linked to chromosomal instability, micronuclei formation, and aneuploidy, contributing to cancer predisposition and developmental disorders. Studies in yeast homologs (e.g., Ctf8) revealed its role in telomere maintenance and replication fidelity. Human CTF18 mutations have been associated with microsatellite instability in colorectal cancers and impaired DNA mismatch repair. Current research focuses on elucidating its interplay with chromatin remodeling, replication stress signaling, and therapeutic targeting in cancer contexts.
×
