| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHST4 |
| Uniprot No | Q8NCG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MAILALFFHMYSHNISSLSMKAQPERMHVLVLSSWRSGSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVFLCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH |
| 分子量 | 66.44 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人碳水化合物硫酸转移酶4(CHST4)的虚构参考文献示例(内容基于典型研究方向构造，非真实文献)：
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1. **文献名称**：**Structural and Functional Characterization of Recombinant Human CHST4 Sulfotransferase**
**作者**：Suzuki T, et al.
**摘要**：本研究利用重组表达系统获得人CHST4蛋白，并通过X射线晶体学解析其三维结构，揭示了其底物结合域的关键氨基酸残基。功能实验表明，CHST4特异性催化碳水化合物6-O-硫酸化，参与选择素配体合成，影响白细胞-内皮细胞黏附。
2. **文献名称**：**CHST4 Expression Correlates with Enhanced Tumor Metastasis in Colorectal Cancer**
**作者**：Hiraoka N, et al.
**摘要**：通过免疫组化分析结肠癌组织，发现CHST4在转移性肿瘤中高表达。实验表明，其介导的硫酸化修饰促进肿瘤细胞表面sialyl Lewis X抗原形成，增强与选择素的结合，进而加速血行转移。
3. **文献名称**：**CHST4 Regulates Lymphocyte Homing via Sulfation of GlyCAM-1**
**作者**：Kumar A, et al.
**摘要**：研究证实CHST4在淋巴结高内皮微静脉中表达，催化GlyCAM-1的硫酸化修饰。基因敲除小鼠模型显示，CHST4缺失导致淋巴细胞归巢障碍，证实其在免疫细胞迁移中的关键作用。
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注：以上文献为示例，实际研究中请通过PubMed或SciHub等平台检索真实论文。如需进一步细化方向(如疾病机制、信号通路)，请补充说明。
Carbohydrate sulfotransferase 4 (CHST4) is a member of the sulfotransferase family that catalyzes the transfer of sulfate groups to carbohydrate moieties, particularly during post-translational modification of glycoproteins and glycolipids. This enzyme specifically sulfates N-acetyllactosamine structures on selectin ligands, such as those found on cell-surface glycoproteins like podocalyxin and CD34. CHST4 plays a critical role in lymphocyte homing and inflammation by generating sulfated epitopes (e.g., 6-sulfo sialyl Lewis X) recognized by L-selectin on leukocytes, facilitating their adhesion and rolling on endothelial cells in high endothelial venules (HEVs) of lymphoid tissues.
Recombinant CHST4 is produced using expression systems (e.g., mammalian or insect cells) to study its enzymatic activity, substrate specificity, and role in immune regulation. Dysregulation of CHST4 has been implicated in pathological conditions, including chronic inflammation, autoimmune diseases, and cancer metastasis, where altered sulfation patterns affect cell-cell interactions and signaling. Research on recombinant CHST4 aids in elucidating its structural-functional relationships, designing inhibitors for therapeutic intervention, and developing biomarkers for disease diagnosis. Its enzymatic products are also explored for applications in glycoengineering and synthetic biology to modulate cellular interactions in targeted therapies.
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