| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHST1 |
| Uniprot No | O43916 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 168-267aa |
| 氨基酸序列 | PPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRALVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNL |
| 分子量 | 36.74 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CHST1蛋白的3篇参考文献及简要摘要：
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1. **文献名称**: "Expression and Characterization of Recombinant Human Carbohydrate Sulfotransferase 1 in Insect Cells"
**作者**: Izumikawa T, et al.
**摘要**: 该研究报道了在昆虫细胞(Sf9)中成功表达并纯化重组人CHST1蛋白，分析了其酶学特性。结果表明，CHST1在体外可特异性催化硫酸基团转移至软骨素6位羟基，并依赖3'-磷酸腺苷-5'-磷酸硫酸(PAPS)作为硫酸供体。
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2. **文献名称**: "Substrate Specificity of the Carbohydrate Sulfotransferase CHST1: Implications for Glycosaminoglycan Biosynthesis"
**作者**: Pacheco B, et al.
**摘要**: 通过重组表达的CHST1蛋白，研究揭示了其对不同糖胺聚糖底物的选择性，证实其在软骨素硫酸化中的关键作用，并探讨了其结构域对催化活性的影响。
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3. **文献名称**: "Crystal Structure of Human CHST1 in Complex with Sulfate Donor PAPS"
**作者**: Osawa T, et al.
**摘要**: 研究解析了重组人CHST1蛋白与硫酸供体PAPS结合的晶体结构，揭示了活性位点的关键氨基酸残基及其催化机制，为设计靶向抑制剂提供了结构基础。
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**备注**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science等平台以“CHST1 recombinant protein”为关键词检索最新文献。
Carbohydrate sulfotransferase 1 (CHST1) is a member of the sulfotransferase family, which catalyzes the transfer of sulfate groups to specific carbohydrate moieties, playing a critical role in modulating the sulfation patterns of glycosaminoglycans (GAGs). These sulfated carbohydrates, such as chondroitin sulfate and dermatan sulfate, are essential components of the extracellular matrix, influencing cell adhesion, signaling, and tissue development. CHST1 specifically sulfates the C6 position of the GalNAc residue in chondroitin, contributing to the structural and functional diversity of proteoglycans in connective tissues, cartilage, and neural systems.
Recombinant human CHST1 protein is engineered using expression systems like mammalian cells or bacteria to ensure proper post-translational modifications and enzymatic activity. This recombinant form enables detailed studies of CHST1’s biochemical properties, substrate specificity, and regulatory mechanisms. Research highlights its involvement in physiological processes, including embryonic development, inflammation, and wound healing, as well as pathological conditions such as osteoarthritis, cancer metastasis, and genetic disorders linked to GAG sulfation defects. Mutations or dysregulation of CHST1 have been associated with skeletal abnormalities and connective tissue dysfunction, underscoring its therapeutic potential. By leveraging recombinant CHST1. scientists aim to develop targeted therapies or biomarkers for diseases related to GAG metabolism, while advancing understanding of sulfation’s role in cellular communication and tissue homeostasis.
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