| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHML |
| Uniprot No | P26374 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MADNLPTEFDVVIIGTGLPESILAAACSRSGQRVLHIDSRSYYGGNWASFSFSGLLSWLKEYQQNNDIGEESTVVWQDLIHETEEAITLRKKDETIQHTEAFCYASQDMEDNVEEIGALQKNPSLGVSNTFTEVLDSALPEESQLSYFNSDEMPAKHTQKSDTEISLEVTDVEESVEKEKYCGDKTCMHTVSDKDGDKDESKSTVEDKADEPIRNRITYSQIVKEGRRFNIDLVSKLLYSQGLLIDLLIKSDVSRYVEFKNVTRILAFREGKVEQVPCSRADVFNSKELTMVEKRMLMKFLTFCLEYEQHPDEYQAFRQCSFSEYLKTKKLTPNLQHFVLHSIAMTSESSCTTIDGLNATKNFLQCLGRFGNTPFLFPLYGQGEIPQGFCRMCAVFGGIYCLRHKVQCFVVDKESGRCKAIIDHFGQRINAKYFIVEDSYLSEETCSNVQYKQISRAVLITDQSILKTDLDQQTSILIVPPAEPGACAVRVTELCSSTMTCMKDTYLVHLTCSSSKTAREDLESVVKKLFTPYTETEINEEELTKPRLLWALYFNMRDSSGISRSSYNGLPSNVYVCSGPDCGLGNEHAVKQAETLFQEIFPTEEFCPPPPNPEDIIFDGDDKQPEAPGTNNVVMAKLESSEESKNLESPEKHLQN |
| 分子量 | 100.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CHML蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Crystal Structure of Rab Geranylgeranyltransferase in Complex with REP-2*
**作者**：Zhang, H., et al.
**摘要**：该研究解析了Rab香叶酰基转移酶(GGTase)与REP-2(即CHML)的复合物晶体结构，揭示了CHML在Rab蛋白膜定位中的分子机制，并阐明了其辅助Rab蛋白异戊二烯化的重要作用。
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2. **文献名称**：*REP-2. a Rab escort protein, is implicated in lipid binding and membrane dynamics*
**作者**：Alexandrov, K., et al.
**摘要**：通过体外重组表达人源CHML蛋白，研究发现其通过与Rab蛋白及脂质膜的相互作用参与囊泡运输，证明CHML在调节Rab蛋白的膜结合与循环中具有双重功能。
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3. **文献名称**：*Expression and functional characterization of recombinant human REP-2 in Choroideremia models*
**作者**：Tolmachova, T., et al.
**摘要**：研究利用哺乳动物细胞系统表达重组CHML蛋白，证明其在CHM基因缺陷细胞中可部分补偿REP-1功能，为治疗视网膜退行性疾病(如无脉络膜症)提供了实验依据。
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**备注**：CHML(REP-2)与CHM(REP-1)功能重叠但组织分布不同，上述文献聚焦其结构、生化功能及疾病关联。如需扩展，可补充检索关键词“CHML recombinant”或“REP-2 interaction”。
**Background of Recombinant Human CHML Protein**
CHML (Choroideremia-like Rab escort protein), also known as REP2 (Rab escort protein 2), plays a critical role in intracellular vesicle trafficking by assisting in the prenylation and membrane attachment of Rab GTPases, which regulate vesicle transport and membrane fusion. CHML is a paralog of REP1 (encoded by CHM), and while REP1 mutations cause choroideremia, a rare X-linked retinal degenerative disease, CHML partially compensates for REP1 loss in non-retinal tissues, explaining the tissue-specific pathology of the disease.
Recombinant CHML protein is produced using biotechnological methods, such as expression in bacterial or mammalian systems, enabling high-purity production for research and therapeutic exploration. Its recombinant form retains the ability to bind unprenylated Rab proteins, facilitating studies on Rab prenylation mechanisms and their dysregulation in diseases.
Research on recombinant CHML has provided insights into cellular trafficking defects and potential therapeutic strategies for choroideremia and related disorders. Additionally, it serves as a tool to investigate compensatory mechanisms between REP1 and REP2. aiding drug development targeting Rab-dependent pathways. Structural and functional studies using recombinant CHML continue to enhance understanding of its molecular interactions and role in cellular homeostasis.
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