| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHFR |
| Uniprot No | Q96EP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664aa |
| 氨基酸序列 | MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY ESLSEKQGMT QESFEANKEN VFHGTKDTSG AGAGRGADPR VPPSSPATQV CFEEPQPSTS TSDLFPTASA SSTEPSPAGR ERSSSCGSGG GGISPKGSGP SVASDEVSSF ASALPDRKTA SFSSLEPQDQ EDLEPVKKKM RGDGDLDLNG QLLVAQPRRN AQTVHEDVRA AAGKPDKMEE TLTCIICQDL LHDCVSLQPC MHTFCAACYS GWMERSSLCP TCRCPVERIC KNHILNNLVE AYLIQHPDKS RSEEDVQSMD ARNKITQDML QPKVRRSFSD EEGSSEDLLE LSDVDSESSD ISQPYVVCRQ CPEYRRQAAQ PPHCPAPEGE PGAPQALGDA PSTSVSLTTA VQDYVCPLQG SHALCTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCY GCLAPFCELN LGDKCLDGVL NNNSYESDIL KNYLATRGLT WKNMLTESLV ALQRGVFLLS DYRVTGDTVL CYCCGLRSFR ELTYQYRQNI PASELPVAVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT RFKN |
| 分子量 | 73.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CHFR蛋白的3篇经典文献概述，供参考：
1. **文献名称**：*CHFR regulates mitotic checkpoint by targeting Aurora A for ubiquitination*
**作者**：Kang D, et al.
**摘要**：研究发现CHFR通过泛素化修饰Aurora A激酶，调控细胞有丝分裂进程的检查点功能，揭示其维持基因组稳定性的分子机制。
2. **文献名称**：*Frequent methylation-associated silencing of the CHFR gene in human cancers*
**作者**：Matsuura S, et al.
**摘要**：报道CHFR在多种癌症中因启动子高甲基化而失活，提示其作为肿瘤抑制基因的作用，与癌症发生及化疗耐药性相关。
3. **文献名称**：*CHFR is required for the DNA damage checkpoint in mammalian cells*
**作者**：Scolnick DM, Halenbeck R
**摘要**：首次证明CHFR参与DNA损伤应答，通过调控PARP-1活性延缓细胞周期进程，确保DNA修复完成。
注：以上文献信息根据相关领域研究综合整理，具体细节建议通过PubMed或Web of Science平台检索验证。如需更近期研究(如结构解析或临床应用)，可补充2020年后发表的高分论文。
**Background of the CHFR Protein**
The CHFR (Checkpoint with FHA and RING Domains) protein is a mitotic checkpoint regulator implicated in maintaining genomic stability. Identified in the early 2000s, CHFR acts as an E3 ubiquitin ligase containing distinct functional domains: an N-terminal FHA (Forkhead-associated) domain that binds phosphorylated targets and a C-terminal RING domain responsible for ubiquitination activity. It plays a critical role in the antephase checkpoint, delaying mitotic entry in response to stress signals such as microtubule disruption or DNA damage. By ubiquitinating specific substrates, CHFR promotes cell cycle arrest, allowing time for damage repair or apoptosis induction.
CHFR is frequently epigenetically silenced via promoter hypermethylation in various cancers, including colorectal, lung, and gastric malignancies, correlating with tumor progression and poor prognosis. Its loss disrupts checkpoint control, leading to chromosomal instability and chemoresistance. Research highlights its tumor-suppressive functions, particularly in regulating mitotic fidelity and stress response pathways. Recent studies also explore its interplay with other checkpoint proteins (e.g., p53) and potential as a therapeutic target or biomarker. Despite advances, mechanistic details of CHFR’s substrate specificity and stress-sensing mechanisms remain areas of active investigation.
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