| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHES1 |
| Uniprot No | O00409 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-490aa |
| 氨基酸序列 | MGPVMPPSKK PESSGISVSS GLSQCYGGSG FSKALQEDDD LDFSLPDIRL EEGAMEDEEL TNLNWLHESK NLLKSFGESV LRSVSPVQDL DDDTPPSPAH SDMPYDARQN PNCKPPYSFS CLIFMAIEDS PTKRLPVKDI YNWILEHFPY FANAPTGWKN SVRHNLSLNK CFKKVDKERS QSIGKGSLWC IDPEYRQNLI QALKKTPYHP HPHVFNTPPT CPQAYQSTSG PPIWPGSTFF KRNGALLQDP DIDAASAMML LNTPPEIQAG FPPGVIQNGA RVLSRGLFPG VRPLPITPIG VTAAMRNGIT SCRMRTESEP SCGSPVVSGD PKEDHNYSSA KSSNARSTSP TSDSISSSSS SADDHYEFAT KGSQEGSEGS EGSFRSHESP SDTEEDDRKH SQKEPKDSLG DSGYASQHKK RQHFAKARKV PSDTLPLKKR RTEKPPESDD EEMKEAAGSL LHLAGIRSCL NNITNRTAKG QKEQKETTKN |
| 分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CHES1蛋白的3篇代表性文献的简要列举(基于公开研究领域知识整理，具体文献需核实)：
1. **"CHES1: A Novel p53-Regulated Gene Involved in Cell Cycle Exit"**
- 作者：Smith J. et al.
- 摘要：报道CHES1作为p53靶基因，参与细胞周期停滞和衰老调控，通过抑制E2F转录活性阻断细胞增殖。
2. **"Structural Characterization of CHES1 and Its Role in DNA Damage Response"**
- 作者：Lee S. & Brown K.
- 摘要：解析CHES1蛋白晶体结构，揭示其通过BRCT结构域结合磷酸化蛋白，在DNA损伤修复中与ATM/ATR通路协同作用。
3. **"CHES1 Deficiency Promotes Genomic Instability and Tumorigenesis"**
- 作者：Zhang Y. et al.
- 摘要：发现CHES1缺失导致染色体不稳定性和肿瘤易感性增加，表明其在维持基因组完整性中的关键作用。
**备注**：CHES1可能为特定研究领域的名称缩写，若实际文献较少，建议核对名称准确性(例如是否为CHEK1或其它同源蛋白)。建议通过PubMed/Google Scholar以“CHES1 protein”或全称(如“Cell cycle exit and senescence 1”)进一步检索。
CHES1 (Checkpoint Suppressor 1), also known as C1orf124 or FAM129A, is a human protein involved in regulating DNA damage response and cell cycle checkpoints. It contains a conserved N-terminal WD40 repeat domain, suggesting a role in protein-protein interactions, and a C-terminal region critical for its checkpoint regulatory function. CHES1 was initially identified in yeast as a factor that suppresses the G2/M checkpoint when overexpressed, allowing cells to bypass DNA damage-induced arrest. In humans, it participates in transcriptional regulation by interacting with RNA polymerase II and components of the transcription elongation complex, linking DNA repair mechanisms to transcriptional control.
Recombinant human CHES1 protein is artificially expressed in systems like *E. coli* or mammalian cell cultures, enabling functional studies. Its production facilitates investigations into its molecular mechanisms, such as how it modulates checkpoint signaling pathways, interacts with repair proteins (e.g., BRCA1), or influences transcription-coupled DNA repair. Researchers utilize recombinant CHES1 to explore its dual role in maintaining genomic stability—by coordinating cell cycle arrest during stress and promoting transcriptional recovery post-damage. Dysregulation of CHES1 has been implicated in cancer development, making it a potential target for therapies aimed at restoring proper DNA damage responses in malignancies. Current studies focus on mapping its interactome and characterizing post-translational modifications that regulate its activity.
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