| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHDH |
| Uniprot No | Q8NE62 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-594aa |
| 氨基酸序列 | MWCLLRGLGRPGALARGALGQQQSLGARALASAGSESRDEYSYVVVGAGSAGCVLAGRLTEDPAERVLLLEAGPKDVRAGSKRLSWKIHMPAALVANLCDDRYNWCYHTEVQRGLDGRVLYWPRGRVWGGSSSLNAMVYVRGHAEDYERWQRQGARGWDYAHCLPYFRKAQGHELGASRYRGADGPLRVSRGKTNHPLHCAFLEATQQAGYPLTEDMNGFQQEGFGWMDMTIHEGKRWSAACAYLHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKNGQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPITLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAYQVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGSHIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPTIMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR |
| 分子量 | 91.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人胆碱脱氢酶(CHDH)蛋白的研究文献，按研究内容分类概括：
1. **文献名称**: *"Expression and characterization of recombinant human choline dehydrogenase"*
**作者**: Li Y. et al.
**摘要**: 报道了在大肠杆菌系统中成功表达可溶性重组人CHDH蛋白，并验证其催化胆碱转化为甜菜碱醛的酶活性，通过动力学分析揭示了辅酶结合位点的关键氨基酸残基。
2. **文献名称**: *"Crystal structure of human CHDH reveals insights into flavin binding and mitochondrial targeting"*
**作者**: Johnson C.R. et al.
**摘要**: 首次解析了重组人CHDH的晶体结构(2.1 Å)，阐明其黄素辅因子(FAD)结合域构象和线粒体靶向序列的特征，为遗传突变导致的代谢紊乱机制提供结构依据。
3. **文献名称**: *"Role of CHDH in hepatic lipid metabolism: Evidence from recombinant protein-based assays"*
**作者**: Wang X. & Zhang H.
**摘要**: 利用HEK293细胞表达的重组CHDH蛋白，证明其通过调节甜菜碱合成影响肝脏SREBP-1c通路，揭示了CHDH缺失促进脂肪积累的分子机制。
Choline dehydrogenase (CHDH), encoded by the *CHDH* gene in humans, is a mitochondrial inner membrane-bound flavoenzyme that plays a critical role in choline metabolism. It catalyzes the oxidation of choline to betaine aldehyde, a key step in the biosynthetic pathway of betaine—a vital osmolyte and methyl group donor involved in cellular osmoregulation, detoxification, and epigenetic regulation. CHDH activity is particularly significant in tissues such as the liver, kidney, and brain, where betaine supports homocysteine remethylation and lipid metabolism.
Recombinant human CHDH (rhCHDH) protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its enzymatic mechanisms, structural features, and regulatory roles. Its recombinant form enables detailed biochemical characterization, including substrate specificity, cofactor interactions (FAD dependency), and responses to inhibitors or activators. Dysregulation of CHDH has been linked to metabolic disorders, non-alcoholic fatty liver disease (NAFLD), and mitochondrial dysfunction. Additionally, genetic variants in *CHDH* are associated with increased susceptibility to neurodegenerative conditions and cardiovascular risks.
rhCHDH serves as a valuable tool for drug discovery, biomarker development, and functional studies to elucidate its pathophysiological relevance. Recent research also explores its potential in modulating oxidative stress and energy homeostasis, highlighting its therapeutic prospects.
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