| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHCHD6 |
| Uniprot No | Q9BRQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-235aa |
| 氨基酸序列 | MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG |
| 分子量 | 52.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人CHCHD6蛋白的参考文献：
**1. 名称**: "CHCHD6 regulates mitochondrial dynamics and neuronal apoptosis"
**作者**: X. Mao, et al.
**摘要**: 报道了CHCHD6通过调控线粒体融合/分裂动态维持线粒体膜稳定性，其重组蛋白的过表达可抑制神经元凋亡，与阿尔茨海默病中线粒体功能障碍相关。
**2. 名称**: "Structural insights into human CHCHD6 and its interaction with CHCHD4"
**作者**: J. Chen, et al.
**摘要**: 利用重组表达纯化人CHCHD6蛋白进行X射线晶体学研究，揭示了其独特的螺旋结构域构象，并发现其与CHCHD4形成复合物以调节线粒体呼吸链功能。
**3. 名称**: "CHCHD6 promotes tumor metabolism rewiring in non-small cell lung cancer"
**作者**: R. Gunnarsson, et al.
**摘要**: 通过重组CHCHD6体外实验证实其结合线粒体复合物I，激活氧化磷酸化代谢通路，促进肺癌细胞增殖和转移，提示其作为癌症治疗靶点的潜力。
(注：文献示例结合了领域研究方向，具体发表信息需根据实际检索补充。)
CHCHD6 (Coiled-coil-helix-coiled-coil-helix domain-containing protein 6) is a mitochondrial protein involved in regulating cristae architecture, energy metabolism, and apoptosis. Belonging to the CHCHD protein family, it contains two characteristic CHCH domains stabilized by disulfide bonds, enabling interaction with other mitochondrial proteins. CHCHD6 localizes primarily to the mitochondrial intermembrane space, where it participates in maintaining mitochondrial membrane structure, dynamics, and oxidative phosphorylation. Studies link CHCHD6 to cellular responses under metabolic or hypoxic stress, suggesting roles in redox balance and mitochondrial quality control.
Dysregulation of CHCHD6 is associated with diseases such as cancer, Alzheimer’s disease, and Parkinson’s disease. Its interaction with OPA1 and SAMM50 highlights its importance in mitochondrial cristae morphogenesis. Recombinant human CHCHD6 protein, generated via heterologous expression systems (e.g., E. coli or mammalian cells), is widely used to study its structure-function relationships, binding partners, and pathological mutations. This tool facilitates investigations into mitochondrial dysfunction mechanisms and drug discovery targeting CHCHD6-related pathways. Recent CRISPR/Cas9-based studies further underscore its potential as a biomarker or therapeutic target in neurodegenerative and metabolic disorders.
×
