| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CGRRF1 |
| Uniprot No | Q99675 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-332aa |
| 氨基酸序列 | FGWDVPVILRNSEETQFSTRVFKKQMRQVKNPFGLEITNPSSASITTGITLTTDCLEDSLLTCYWGCNVQKLYEALQKHVYCFRISTPQALEDALYSEYLYQEQYFIKKDSKEEIYCQLPRDTKIEDFGTVPRSRYPLVALLTLADEDDREIYDIISMVSVIHIPDRTYKLSCRILYQYLLLAQGQFHDLKQLFMSANNNFTPSNNSSSEEKNTDRSLLEKVGLSESEVEPSEENSKDCVVCQNGTVNWVLLPCRHTCLCDGCVKYFQQCPMCRQFVQESFALCSQKEQDKDKPKTL |
| 分子量 | 58.41 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CGRRF1蛋白的参考文献示例(注：以下文献为模拟内容，实际文献需通过学术数据库验证)：
1. **文献名称**：**"CGRRF1 regulates cell proliferation by modulating ubiquitin-mediated degradation of cyclin D1"**
**作者**：Chen et al., 2016
**摘要**：本研究利用重组人CGRRF1蛋白发现其作为E3泛素连接酶，靶向细胞周期蛋白Cyclin D1促进其泛素化降解，从而抑制细胞增殖。通过体外实验证实了CGRRF1与Cyclin D1的相互作用，为癌症治疗提供了机制见解。
2. **文献名称**：**"Recombinant expression and functional analysis of CGRRF1 in hepatocellular carcinoma"**
**作者**：Liu et al., 2019
**摘要**：文章报道了重组CGRRF1蛋白在肝细胞癌中的功能，发现其过表达抑制肿瘤细胞迁移和侵袭，并通过调控TGF-β通路影响EMT过程，提示其作为肝癌潜在抑癌基因的作用。
3. **文献名称**：**"Structural insights into the RING domain of human CGRRF1 by recombinant protein crystallography"**
**作者**：Kim et al., 2017
**摘要**：通过重组表达并解析CGRRF1蛋白的RING结构域晶体结构，揭示了其与泛素结合酶(E2)相互作用的分子机制，为设计靶向CGRRF1的小分子药物奠定结构基础。
4. **文献名称**：**"CGRRF1 modulates oxidative stress response via NRF2 ubiquitination"**
**作者**：Gupta et al., 2021
**摘要**：研究发现重组CGRRF1蛋白通过促进转录因子NRF2的泛素化降解，加剧氧化应激诱导的细胞损伤，揭示了其在氧化应激相关疾病(如神经退行性疾病)中的调控作用。
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**备注**：以上内容为基于CGRRF1蛋白已知功能(如泛素化调控、癌症关联性等)的模拟文献，实际研究请通过PubMed、Web of Science等平台检索关键词“CGRRF1”、“RNF197”(其别名)及“recombinant”获取。建议结合最新综述或高通量筛选研究扩展文献来源。
**Background of Human CGRRF1 Protein**
Cell Growth Regulating Ring Finger Protein 1 (CGRRF1) is a ubiquitously expressed protein encoded by the *CGRRF1* gene, located on human chromosome 14q22.1. It belongs to the RING finger protein family, characterized by a conserved C3H2C3-type RING domain that confers E3 ubiquitin ligase activity. This enzymatic function enables CGRRF1 to participate in ubiquitination, a post-translational modification process critical for regulating protein degradation, trafficking, and signaling.
CGRRF1 is implicated in modulating cell growth, apoptosis, and stress responses. Studies suggest it interacts with components of the ubiquitin-proteasome system, potentially targeting substrates involved in cell cycle control or DNA repair. While its exact physiological role remains under investigation, CGRRF1 has been linked to pathological conditions, including cancers (e.g., hepatocellular carcinoma), where dysregulated expression correlates with tumor progression. Additionally, research highlights its potential involvement in neurodegenerative and autoimmune disorders, possibly through immune modulation or oxidative stress pathways.
Experimental models, including knockout mice, reveal that CGRRF1 deficiency may alter cellular proliferation and stress adaptation. Despite these advances, the full spectrum of its interacting partners, downstream targets, and tissue-specific functions requires further elucidation. Understanding CGRRF1’s molecular mechanisms could provide insights into therapeutic strategies for diseases linked to ubiquitination dysregulation.
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