| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CGI-51 |
| Uniprot No | Q9Y512 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-468aa |
| 氨基酸序列 | MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSMHSIGPQSEGDYLGGEAYWAGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL |
| 分子量 | 77.22 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CGRRF1蛋白的假设性参考文献示例(实际文献需通过学术数据库检索确认)：
1. **文献名称**：**"CGRRF1 regulates apoptosis via ubiquitination of Bcl-2 in cancer cells"**
**作者**：Park et al. (2018)
**摘要**：研究揭示CGRRF1作为E3泛素连接酶，通过促进Bcl-2蛋白的泛素化降解，增强癌细胞对凋亡的敏感性，为靶向癌症治疗提供潜在机制。
2. **文献名称**：**"Cloning and functional characterization of human CGRRF1 as a novel regulator in the ubiquitin-proteasome pathway"**
**作者**：Li et al. (2015)
**摘要**：首次报道人CGRRF1重组蛋白的基因克隆与原核表达，证实其通过RING结构域介导泛素化反应，并调控细胞周期进程。
3. **文献名称**：**"CGRRF1 modulates autophagy by interacting with ATG16L1 in neurodegenerative disease models"**
**作者**：Zhang et al. (2021)
**摘要**：发现CGRRF1通过与自噬相关蛋白ATG16L1相互作用，调控神经细胞中错误折叠蛋白的清除，敲除CGRRF1导致自噬通量异常和神经元死亡。
4. **文献名称**：**"Structural insights into the RING domain of CGRRF1 and its role in substrate recognition"**
**作者**：Wang & Chen (2020)
**摘要**：通过X射线晶体学解析CGRRF1的RING结构域三维结构，揭示其与泛素结合酶(E2)的相互作用界面，为泛素化底物特异性提供结构基础。
**注**：以上为假设文献，实际研究需查阅权威数据库(如PubMed、Web of Science)。建议结合关键词“CGRRF1/RNF141”、“ubiquitination”、“autophagy”等检索最新文献。
Recombinant human CGRRF1 (Cell Growth Regulatory Ring Finger Protein 1) is a protein encoded by the CGRRF1 gene located on chromosome 14q22.1. It belongs to the RING finger protein family, characterized by a conserved C3HC4-type zinc-binding RING domain, which is critical for mediating protein-protein interactions and conferring E3 ubiquitin ligase activity. CGRRF1 is implicated in regulating cellular processes such as apoptosis, proliferation, and the ubiquitin-proteasome system, which governs protein degradation and homeostasis. Studies suggest its involvement in modulating pathways like the NF-κB signaling cascade and stress response mechanisms, potentially linking it to inflammatory diseases and cancer progression. The recombinant form of CGRRF1 is typically produced via expression systems (e.g., E. coli or mammalian cells) for functional studies, enabling researchers to explore its biochemical properties, interactions, and role in disease models. Its dysregulation has been observed in certain cancers, neurodegenerative conditions, and immune disorders, positioning it as a potential therapeutic target. Ongoing research aims to clarify its precise molecular mechanisms and therapeutic relevance.
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