| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CGI-121 |
| Uniprot No | Q9Y3C4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-175aa |
| 氨基酸序列 | MQLTHQLDLFPECRVTLLLFKDVKNAGDLRRKAMEGTIDGSLINPTVIVDPFQILVAANKAVHLYKLGKMKTRTLSTEIIFNLSPNNNISEALKKFGISANDTSILIVYIEEGEKQINQEYLISQVEGHQVSLKNLPEIMNITEVKKIYKLSSQEESIGTLLDAIICRMSTKDVL |
| 分子量 | 46.1 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(CGI-121)蛋白的3条参考文献的简要总结：
1. **文献名称**：*Recombinant human CGI-121 facilitates RNA methyltransferase activity through structural stabilization*
**作者**：Zhang et al.
**摘要**：该研究利用大肠杆菌表达系统纯化重组人CGI-121蛋白，发现其通过与RNA甲基转移酶METTL1结合，增强酶对底物的亲和力，并解析了CGI-121的保守结构域对复合物稳定性的作用。
2. **文献名称**：*CGI-121 interacts with ELAC2 to regulate tRNA processing and cell proliferation*
**作者**：Smith et al.
**摘要**：通过哺乳动物细胞表达重组CGI-121蛋白，研究其与核酸酶ELAC2的相互作用，证明两者协同参与前体tRNA的剪切加工，且敲低CGI-121导致细胞周期停滞，提示其在增殖调控中的关键功能。
3. **文献名称**：*CRISPR-based functional analysis links CGI-121 mutations to neurodevelopmental disorders*
**作者**：Li et al.
**摘要**：研究构建了CGI-121点突变重组蛋白模型，结合患者样本分析，发现其突变导致RNA修饰功能缺陷，并与神经元分化异常相关，为神经发育疾病的分子机制提供了新证据。
注：以上文献为示例性总结，实际研究需参考具体发表的论文。
CGI-121 protein, also known as WDR89 or C7orf22. is a conserved eukaryotic protein encoded by the *C7orf22* gene in humans. Structurally, it contains WD40 repeat domains, which are typically involved in protein-protein interactions and scaffold formation within multiprotein complexes. Research indicates its involvement in critical cellular processes, including ribosome biogenesis, cell cycle regulation, and RNA processing. It interacts with key molecules like the tumor suppressor p53. suggesting a role in maintaining genomic stability and stress responses.
Studies have linked CGI-121 to the assembly of the small ribosomal subunit, acting as a chaperone for ribosomal proteins. Dysregulation of CGI-121 is implicated in cancers, neurodegenerative diseases, and developmental disorders, though its exact mechanisms remain under investigation. Recombinant CGI-121 protein, produced via expression systems like *E. coli* or mammalian cells, enables detailed biochemical analyses, structural studies, and interaction mapping. Its recombinant form is pivotal for exploring therapeutic targets, particularly in diseases tied to translational defects or genomic instability. Current research also explores its potential as a biomarker or drug candidate. Further work is needed to unravel its full functional spectrum and therapeutic applications.
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