| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CGB5 |
| Uniprot No | P0DN86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MEMFQGLLLLLLLSMGGTWASKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPTMTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDCGGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ |
| 分子量 | 43.89 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CGB5蛋白的3篇参考文献示例(基于领域研究趋势构造的概括性内容，非真实文献)：
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1. **文献名称**: *"Expression and Purification of Recombinant Human CGB5 in HEK293 Cells: Implications for Hormone Activity Studies"*
**作者**: Smith et al. (2020)
**摘要**: 研究报道了在HEK293哺乳动物细胞系统中成功表达重组人CGB5蛋白，并通过亲和层析纯化获得高纯度蛋白。验证了其与黄体生成素/绒毛膜促性腺激素(LH/CG)受体结合的能力，证实其体外生物活性可用于生殖激素相关研究。
2. **文献名称**: *"CGB5 Overexpression in Non-Small Cell Lung Cancer: A Potential Diagnostic Biomarker"*
**作者**: Lee et al. (2018)
**摘要**: 探讨了CGB5在肺癌组织中的异常表达，发现重组CGB5蛋白可通过特异性抗体检测到其在肿瘤细胞中的高表达，提示其可能作为肺癌早期诊断的血清标志物，并参与肿瘤细胞增殖调控。
3. **文献名称**: *"Structural Characterization of Recombinant CGB5 and Its Role in Placental Development"*
**作者**: Zhang et al. (2015)
**摘要**: 利用X射线晶体学解析了重组CGB5蛋白的三维结构，揭示其与天然hCG的β亚基高度相似。功能实验表明CGB5在胎盘形成中促进滋养层细胞分化，可能为妊娠相关疾病治疗提供靶点。
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**注**：以上内容为模拟构造的示例文献，实际研究中建议通过PubMed、Web of Science等平台检索关键词(如“recombinant CGB5”、“human chorionic gonadotropin beta 5”)获取真实发表文献。
Recombinant human chorionic gonadotropin beta subunit 5 (CGB5) protein is a synthetic form of a critical subunit of human chorionic gonadotropin (hCG), a hormone essential for pregnancy maintenance. Naturally, hCG is a heterodimeric glycoprotein composed of an alpha subunit (shared with other hormones like LH, FSH, and TSH) and a unique beta subunit encoded by the CGB gene cluster. The CGB gene family includes six homologous genes (CGB1-9. with some genes numbered non-sequentially), of which CGB5 is highly expressed in placental trophoblast cells and contributes to the majority of β-hCG isoforms during early gestation.
CGB5-derived β-hCG supports embryonic implantation, trophoblast proliferation, and maternal immunotolerance by binding to LH/hCG receptors, triggering cAMP signaling. Recombinant CGB5 protein, typically produced in mammalian or bacterial expression systems, retains these biological functions. It is utilized in research to study pregnancy disorders, infertility, and cancers, as ectopic hCG expression is linked to malignancies like choriocarcinoma and bladder cancer. Additionally, recombinant CGB5 serves as a reference standard in diagnostic assays for pregnancy monitoring and tumor detection. Its engineered production ensures high purity and consistency, overcoming limitations of urinary-derived hCG. Ongoing studies explore its therapeutic potential in reproductive medicine and oncology.
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