| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEP112 |
| Uniprot No | Q8N8E3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MWASLSLDHPSAKENQALRLIEMREENGNVPKTEQAGSLKPLRDTGKSNLKEKKANSKLKQIEKEYTQKLAKSSQIIAELQTTISSLKEENSQQQLAAERRLQDVRQKFEDEKKQLIRDNDQAIKVLQDELENRSNQVRCAEKKLQHKELESQEQITYIRQEYETKLKGLMPASLRQELEDTISSLKSQVNFLQKRASILQEELTTYQGRR |
| 分子量 | 50.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CEP112蛋白的模拟参考文献示例(仅供参考)：
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1. **Title**: *CEP112 regulates centrosome maturation by promoting PLK1 recruitment*
**Authors**: Smith J, Lee K, et al.
**Summary**: 该研究揭示了CEP112通过结合PLK1激酶调控中心体成熟的机制，并利用重组CEP112蛋白验证其在细胞周期中的功能缺失导致纺锤体异常。
2. **Title**: *Structural insights into human CEP112 dynamics during interphase*
**Authors**: Chen R, Tanaka H, et al.
**Summary**: 通过重组CEP112蛋白的晶体结构分析，阐明了其在细胞间期的构象变化，并发现其C端结构域对维持中心体稳定性至关重要。
3. **Title**: *Recombinant CEP112 facilitates DNA damage response in cancer cells*
**Authors**: Gupta S, Wang Y, et al.
**Summary**: 实验表明，重组CEP112蛋白可通过促进ATM/ATR信号通路增强癌细胞对辐射的耐受性，提示其在肿瘤治疗中的潜在靶点作用。
4. **Title**: *Development of a high-yield CEP112 protein expression system in E. coli*
**Authors**: Müller P, Schmidt A.
**Summary**: 优化了重组人CEP112蛋白在大肠杆菌中的可溶性表达工艺，并验证了纯化蛋白在体外微管成核实验中的生物活性。
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**注**：以上文献为模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献。
Centrosomal protein 112 (CEP112) is a critical component of the centrosome, a microtubule-organizing center essential for cell division, cytoskeletal organization, and cilia formation. It plays a role in maintaining centrosomal integrity, regulating the cell cycle, and ensuring proper spindle orientation during mitosis. CEP112 interacts with other centrosomal proteins, such as CEP170 and CEP290. contributing to the recruitment of regulatory molecules involved in microtubule dynamics and checkpoint signaling. Its dysfunction has been linked to genomic instability, a hallmark of cancer, and ciliopathies—genetic disorders affecting cilia structure or function, such as Joubert syndrome and nephronophthisis.
Recombinant human CEP112 protein is engineered using expression systems (e.g., bacterial or mammalian cells) to produce purified, biologically active forms for functional studies. Researchers employ it to investigate CEP112’s role in centrosome amplification, mitotic errors, or ciliogenesis via knockdown/overexpression assays, protein-protein interaction mapping, and structural analyses. Its applications extend to screening therapeutic agents targeting centrosome-related pathologies. Mutations or aberrant expression of CEP112 are implicated in tumorigenesis and developmental defects, making it a potential biomarker or therapeutic target. Further studies aim to elucidate its precise mechanisms in disease contexts.
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