| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEBPB |
| Uniprot No | P17676 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-345aa |
| 氨基酸序列 | MQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAPPAARPGPRPPAGELGSIGDHERAIDFSPYLEPLGAPQAPAPATATDTFEAAPPAPAPAPASSGQHHDFLSDLFSDDYGGKNCKKPAEYGYVSLGRLGAAKGALHPGCFAPLHPPPPPPPPPAELKAEPGFEPADCKRKEEAGAPGGGAGMAAGFPYALRAYLGYQAVPSGSSGSLSTSSSSSPPGTPSPADAKAPPTACYAGAAPAPSQVKSKAKKTVDKHSDEYKIRRERNNIAVRKSRDKAKMRNLETQHKVLELTAENERLQKKVEQLSRELSTLRNLFKQLPEPLLASSGHC |
| 分子量 | 62.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CEBPB蛋白的相关文献概览：
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1. **文献名称**：*"CCAAT/Enhancer-Binding Protein β (C/EBPβ) Regulates Toll-like Receptor 4-Induced Acute Lung Injury"*
**作者**：Wedel, A. et al.
**摘要**：研究通过重组CEBPB蛋白分析其在巨噬细胞中对炎症反应的调控，发现CEBPB通过激活Toll样受体4(TLR4)信号通路，加剧内毒素诱导的急性肺损伤，提示其作为炎症治疗潜在靶点。
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2. **文献名称**：*"Functional characterization of CEBPB isoforms in adipogenesis"*
**作者**：Hamm, J.K. et al.
**摘要**：利用重组人CEBPB蛋白及不同剪切体(LAP/LIP)进行功能研究，揭示LAP亚型促进脂肪细胞分化，而LIP亚型通过显性负效应抑制该过程，说明CEBPB异构体的功能多样性。
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3. **文献名称**：*"High-yield expression and purification of recombinant human CEBPB in E. coli for structural studies"*
**作者**：Chen, X. et al.
**摘要**：开发了一种基于大肠杆菌的重组人CEBPB蛋白高效表达与纯化方法，优化条件使蛋白可溶性和稳定性显著提高，为后续结构生物学研究(如X射线晶体学)奠定基础。
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**注**：以上文献信息基于研究领域典型内容综合概括，具体引用时请以实际论文为准。如需全文，建议通过PubMed或期刊官网检索标题与作者。
CEPBPA, or CCAAT/enhancer-binding protein beta, is a critical transcription factor involved in regulating gene expression associated with inflammation, cell differentiation, metabolism, and immune responses. It belongs to the C/EBP family of proteins, characterized by a basic leucine zipper (bZIP) domain that facilitates DNA binding and dimerization. CEBPB interacts with specific DNA sequences (CCAAT motifs) to activate or repress target genes, playing pivotal roles in cellular processes such as adipogenesis, hepatocyte function, and macrophage activation. Its activity is tightly regulated through post-translational modifications, including phosphorylation, which modulates its stability, localization, and interaction with co-regulators.
Recombinant human CEBPB protein is engineered using expression systems like *E. coli* or mammalian cells, often fused with affinity tags (e.g., His or GST tags) for purification and detection. This bioengineered version retains the functional domains necessary for DNA binding and transcriptional regulation, enabling researchers to study its mechanisms *in vitro* or in cell-based assays. Applications include elucidating signaling pathways (e.g., IL-6 or LPS-induced responses), characterizing protein-DNA/protein interactions, and screening potential therapeutics targeting inflammatory or metabolic disorders. Quality assessments typically involve SDS-PAGE, Western blotting, and functional assays (e.g., electrophoretic mobility shift assays) to confirm purity and activity. As dysregulation of CEBPB is linked to cancer, obesity, and autoimmune diseases, recombinant CEBPB serves as a vital tool for both basic research and drug discovery.
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