| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDKL3 |
| Uniprot No | Q8IVW4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-592aa |
| 氨基酸序列 | MEMYETLGKVGEGSYGTVMKCKHKNTGQIVAIKIFYERPEQSVNKIAMREIKFLKQFHHENLVNLIEVFRQKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAIDYLHSNNIIHRDIKPENILVSQSGITKLCDFGFARTLAAPGDIYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLLHKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLADIVHACLQIDPADRISSSDLLHHEYFTRDGFIEKFMPELKAKLLQEAKVNSLIKPKESSKENELRKDERKTVYTNTLLSSSVLGKEIEKEKKPKEIKVRVIKVKGGRGDISEPKKKEYEGGLGQQDANENVHPMSPDTKLVTIEPPNPINPSTNCNGLKENPHCGGSVTMPPINLTNSNLMAANLSSNLFHPSVRLTERAKKRRTSSQSIGQVMPNSRQEDPGPIQSQMEKGIFNERTGHSDQMANENKRKLNFSRSDRKEFHFPELPVTIQSKDTKGMEVKQIKMLKRESKKTESSKIPTLLNVDQNQEKQEGGDGHCEGKNLKRNRFFFW |
| 分子量 | 93.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDKL3蛋白的示例性参考文献(内容为假设性概括，供参考)：
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1. **文献名称**：*"CDKL3: A novel cyclin-dependent kinase-like protein involved in cell cycle regulation"*
**作者**：Malumbres et al. (2001)
**摘要**：首次克隆并鉴定CDKL3作为细胞周期素依赖性激酶(CDK)家族新成员，发现其与CDK1和CDK5具有序列同源性，可能在G1/S期调控中发挥作用，并在脑组织中高表达。
2. **文献名称**：*"CDKL3 promotes tumor cell proliferation via phosphorylation of Rb protein in colorectal cancer"*
**作者**：Ferguson et al. (2015)
**摘要**：研究显示CDKL3在结直肠癌中异常高表达，可通过磷酸化视网膜母细胞瘤蛋白(Rb)驱动肿瘤细胞增殖，敲低CDKL3显著抑制癌细胞生长。
3. **文献名称**：*"Structural and functional characterization of CDKL3 kinase domain reveals unique ATP-binding properties"*
**作者**：Li & Wang (2018)
**摘要**：解析CDKL3激酶结构域晶体结构，揭示其ATP结合口袋的独特构象，并通过体外激酶实验证明其对组蛋白H1的磷酸化活性依赖MAPK信号通路。
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**提示**：CDKL3相关研究较少，实际文献需通过PubMed、Web of Science等平台查询。部分论文可能仅间接涉及CDKL3或与其他CDK家族蛋白对比分析。建议结合关键词“CDKL3 kinase”“CDKL3 cancer”或“CDKL3 structure”检索最新研究。
CDKL3 (cyclin-dependent kinase-like 3) is a serine/threonine protein kinase belonging to the CDKL family, which shares structural similarities with cyclin-dependent kinases (CDKs) but lacks direct cyclin-binding motifs. Encoded by the *CDKL3* gene in humans, this enzyme is implicated in cell cycle regulation, signal transduction, and cellular differentiation. While its precise biological mechanisms remain less defined compared to other family members like CDKL5 (associated with neurodevelopmental disorders), CDKL3 is thought to phosphorylate substrates involved in transcriptional regulation and chromatin remodeling.
Expression of CDKL3 is observed in various tissues, notably the brain, testis, and proliferating cells, suggesting roles in neural function and germ cell development. Studies link it to cancer progression, with elevated levels reported in hepatocellular carcinoma and gliomas, potentially influencing tumor cell proliferation or survival pathways. Structural analyses reveal conserved kinase domains, yet its activation mechanisms and substrate specificity require further elucidation.
Recombinant CDKL3 protein is utilized in biochemical assays to study enzymatic activity, substrate interactions, and inhibitor screening. Despite advances, functional redundancy within the kinase family and limited in vivo data pose challenges in delineating its unique physiological roles. Ongoing research aims to clarify its contribution to developmental processes and disease pathogenesis, offering potential therapeutic targets for cancers or neurological conditions.
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