| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDCA1 |
| Uniprot No | Q9BZD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | METLSFPRYNVAEIVIHIRNKILTGADGKNLTKNDLYPNPKPEVLHMIYMRALQIVYGIRLEHFYMMPVNSEVMYPHLMEGFLPFSNLVTHLDSFLPICRVNDFETADILCPKAKRTSRFLSGIINFIHFREACRETYMEFLWQYKSSADKMQQLNAAHQEALMKLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNISEKTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKNARQEVVEKYEIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKLKTEENSFKRLMIVKKEKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEKRGAVYERVTTINQEIQKIKLGIQQLKDAAEREKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDEKTAELKRKMFKMST |
| 分子量 | 76.78 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDCA1蛋白的参考文献示例(均为虚构，仅用于演示格式)：
1. **文献名称**: *Recombinant human CDCA1 promotes mitotic progression by stabilizing kinetochore-microtubule attachments*
**作者**: Yamada R, et al.
**摘要**: 通过昆虫细胞表达系统制备重组CDCA1蛋白，研究发现其通过稳定动粒-微管连接调控有丝分裂进程，缺失CDCA1导致染色体错配。
2. **文献名称**: *CDCA1 interacts with Aurora B kinase: Implications for cancer therapeutic targets*
**作者**: Smith J, et al.
**摘要**: 利用重组CDCA1蛋白进行体外结合实验，证实其与Aurora B激酶相互作用，抑制该通路可减少肿瘤细胞增殖，提示其作为治疗靶点的潜力。
3. **文献名称**: *Development of a CDCA1-based ELISA for early detection of lung adenocarcinoma*
**作者**: Chen L, et al.
**摘要**: 通过大肠杆菌表达重组CDCA1蛋白并制备单克隆抗体，建立ELISA检测法，发现肺癌患者血清中CDCA1水平显著升高，与早期诊断相关。
4. **文献名称**: *Structural insights into CDCA1-DNA interaction using recombinant protein crystallography*
**作者**: Tanaka K, et al.
**摘要**: 解析重组CDCA1蛋白的晶体结构，揭示其DNA结合域的关键氨基酸残基，为研究其在基因组稳定性中的作用提供分子基础。
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**备注**：上述文献及作者为假设示例，实际研究中CDCA1可能与动粒复合体(如NUF2)相关。建议通过PubMed或Google Scholar以“CDCA1 recombinant”或“NUF2 recombinant”检索真实文献。
CDCA1 (Cell Division Cycle Associated 1), also known as ANTIGEN NY-CO-45. is a human protein encoded by the *CDCA1* gene. It plays a critical role in regulating cell cycle progression, particularly during mitosis. CDCA1 is involved in spindle assembly checkpoint control, ensuring accurate chromosome segregation by monitoring microtubule-kinetochore attachments. Dysregulation of CDCA1 has been linked to chromosomal instability, a hallmark of cancer. Overexpression of CDCA1 is observed in various malignancies, including lung, breast, and colorectal cancers, and correlates with poor prognosis, suggesting its potential as a therapeutic target or diagnostic biomarker.
Recombinant human CDCA1 protein is typically produced using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. Purification methods often employ affinity tags (e.g., His-tag) followed by chromatography. The recombinant protein is widely used in functional studies to investigate its interactions with mitotic regulators (e.g., Aurora kinases, PLK1), apoptosis pathways, and DNA damage responses. It also facilitates structural analysis, antibody development, and high-throughput drug screening for CDCA1-targeted therapies. Current research focuses on understanding its dual roles in tumorigenesis and normal cell division, as well as exploring strategies to modulate its activity in cancer treatment while minimizing off-target effects on healthy tissues.
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