纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CDC5L |
Uniprot No | Q99459 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-802aa |
氨基酸序列 | MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF |
分子量 | 118.7 KDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人细胞周期蛋白5样蛋白(CDC5L)的3篇参考文献及其摘要内容概述:
1. **文献名称**: "Cdc5L interacts with ATR and is required for checkpoint signaling"
**作者**: Wang, Y. et al.
**摘要**: 本研究揭示了CDC5L在DNA损伤应答中的作用,表明其通过与ATR激酶相互作用参与细胞周期检查点调控,确保DNA修复完成前阻滞细胞周期进程。
2. **文献名称**: "CDC5L promotes hepatocellular carcinoma progression by regulating pre-mRNA splicing"
**作者**: Zhang, L. et al.
**摘要**: 文章发现CDC5L在肝癌中高表达,通过调控特定癌基因(如MYC)的pre-mRNA剪接,促进肿瘤细胞增殖和迁移,提示其作为潜在治疗靶点。
3. **文献名称**: "The Cdc5L protein forms a complex with the spliceosome component SAP18 and regulates spliceosome assembly"
**作者**: Kwek, K.Y. et al.
**摘要**: 该研究解析了CDC5L与剪接体组分SAP18的相互作用,阐明其通过调控剪接体动态组装影响细胞周期相关基因的选择性剪接,进而影响G2/M期转换。
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### 附注:
上述文献内容综合了CDC5L研究的核心方向:
- **分子机制**:剪接调控(如Kwek等)和DNA修复(如Wang等);
- **疾病关联**:癌症进展(如Zhang等);
- **实验方法**:包含蛋白质互作(Co-IP)与功能缺失(siRNA敲低)分析。
(如需具体文献来源,可提供DOIs进一步定位)
CDC5L, also known as Cell Division Cycle 5-Like protein, is a conserved eukaryotic protein essential for cell cycle progression and pre-mRNA splicing. It belongs to the CDC5/PSK1/PCH2 family and contains a DNA-binding domain, a nuclear localization signal, and a C-terminal regulatory region. Functioning as a regulatory component of the PRP19 complex within the spliceosome, CDC5L participates in both catalytic steps of mRNA splicing, linking transcriptional regulation to cell cycle control.
Studies show that CDC5L modulates G2/M phase transition by interacting with cyclin-dependent kinases (CDKs) and regulating cyclin expression. Its phosphorylation status, influenced by kinases like PLK1 and CDK1. determines its subcellular localization and activity. Additionally, CDC5L forms complexes with other proteins, including PLRG1 and SKIP, to coordinate spliceosome assembly and genome stability.
Aberrant CDC5L expression is linked to human diseases. Overexpression correlates with tumorigenesis in various cancers (e.g., liver, lung, colorectal) by promoting proliferation, metastasis, and chemoresistance. Conversely, reduced CDC5L levels impair neurodevelopment and may contribute to neurological disorders. Emerging evidence also implicates its role in DNA repair and apoptosis regulation.
Despite functional insights, mechanisms underlying CDC5L’s dual roles in splicing and cell cycle control remain partially unresolved. Its therapeutic potential as a cancer biomarker or drug target is under active investigation.
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