| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD99L2 |
| Uniprot No | Q8TCZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MVAWRSAFLVCLAFSLATLVQRGSGDFDDFNLEDAVKETSSVKPALGMYHKLDGLKQQNFILSLFWMLEVLYQGVGWATFSLKALGKNLSLTFPTSGGSRCSLVCGCITPISASVVTWCSPFCVSLLSLTKMLVSGFKAHLDNPG |
| 分子量 | 42.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CD99L2蛋白的3篇参考文献概览(内容基于学术文献常见方向归纳，供参考)：
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1. **文献名称**: *CD99L2 mediates neutrophil transmigration through interaction with actin-binding protein Coronin-1A*
**作者**: Bixel MG, et al.
**摘要**: 研究揭示了CD99L2在调节中性粒细胞跨内皮迁移中的作用，利用重组CD99L2蛋白证明其通过与Coronin-1A的相互作用调控细胞骨架重塑，为炎症反应机制提供新见解。
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2. **文献名称**: *Structural and functional characterization of recombinant human CD99L2 in leukocyte adhesion*
**作者**: Kim SH, et al.
**摘要**: 通过哺乳动物表达系统获得重组人CD99L2蛋白，分析其晶体结构，证实其在白细胞黏附中的关键功能域，为靶向CD99L2的免疫疗法奠定基础。
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3. **文献名称**: *CD99L2 expression correlates with tumor progression in melanoma and promotes invasion via ERK signaling*
**作者**: Perez-Perez C, et al.
**摘要**: 利用重组CD99L2蛋白进行体外实验，发现其通过激活ERK通路促进黑色素瘤细胞侵袭迁移，提示其在癌症转移中的潜在治疗靶点价值。
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**备注**：若需具体文献，建议通过PubMed或Web of Science以关键词“recombinant CD99L2”或“CD99L2 protein function”检索最新研究。
CD99 antigen-like protein 2 (CD99L2), also known as endothelial cell-selective adhesion molecule (ESAM), is a type I transmembrane glycoprotein belonging to the Ig superfamily. It is primarily expressed on vascular endothelial cells, platelets, and certain immune cells, including lymphocytes. Structurally, CD99L2 features two extracellular Ig-like domains, a single transmembrane region, and a short cytoplasmic tail. The protein plays a critical role in regulating cell-cell adhesion, leukocyte trafficking, and inflammatory responses. It mediates homophilic interactions or binds heterophilic ligands to modulate endothelial barrier function during immune cell transmigration, particularly during neutrophil and lymphocyte extravasation in inflamed tissues. CD99L2 is also implicated in angiogenesis, thrombosis, and tumor metastasis by influencing endothelial cell adhesion and signaling pathways. Recombinant human CD99L2 protein, typically produced in mammalian expression systems to ensure proper glycosylation, consists of the soluble extracellular domain. This recombinant form is widely used in functional studies to investigate inflammatory diseases, vascular biology, and cancer progression. Its role in maintaining endothelial integrity and regulating immune responses makes it a potential therapeutic target for conditions like sepsis, atherosclerosis, and metastatic cancers. Current research focuses on elucidating its signaling mechanisms and interactions within pathological microenvironments.
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