| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD3Z |
| Uniprot No | P20963 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 62-164aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSRVKFSRSADAPAYQQGQNQLYNELNLG RREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGM KGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR |
| 分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CD3ζ(CD3Z)蛋白的3篇参考文献的简要归纳：
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1. **文献名称**：**"Assembly of the TCR-CD3 Complex: Structural Analysis of the CD3ζ Homodimer"**
**作者**：Call, M.E. 等 (2002)
**摘要**：该研究解析了CD3ζ链在TCR-CD3复合体中的结构功能，通过重组表达人CD3ζ蛋白并结合X射线晶体学，揭示了其同源二聚化结构域的关键作用，阐明其如何参与T细胞受体信号转导。
2. **文献名称**：**"Chimeric Antigen Receptor T Cells Employing TCR ζ-Coupled Signaling Domains"**
**作者**：Sadelain, M. 等 (2010)
**摘要**：探讨了将重组人CD3ζ链的胞内信号结构域(含ITAMs)整合至CAR-T细胞的设计，验证其体外激活T细胞杀伤肿瘤的效果，为后续CAR-T疗法开发奠定基础。
3. **文献名称**：**"Production and Functional Analysis of Recombinant Soluble CD3ζ for Immunotherapy Development"**
**作者**：Liu, Y. 等 (2015)
**摘要**：描述了在大肠杆菌中高效表达可溶性重组CD3ζ蛋白的优化策略，通过体外实验证明该蛋白可与TCR复合物结合，并潜在用于调节T细胞活性的治疗分子。
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以上文献涵盖结构生物学、基因工程治疗和蛋白生产领域，均涉及重组CD3ζ蛋白的功能或应用。如需具体期刊信息或补充文献，可进一步提供关键词或研究背景。
CD3ζ (CD3Z) is a critical subunit of the T-cell receptor (TCR) complex, essential for T-cell activation and adaptive immune responses. It is encoded by the CD3Z gene in humans and forms part of the CD3 heterodimer (alongside CD3γ, δ, and ε subunits) non-covalently associated with the TCR α/β chains. Structurally, CD3ζ contains a short extracellular domain, a transmembrane region, and a cytoplasmic tail with three immunoreceptor tyrosine-based activation motifs (ITAMs). These ITAMs are phosphorylated upon TCR engagement with antigen-presenting cells, initiating downstream signaling cascades that drive T-cell proliferation, cytokine production, and cytotoxic activity.
Recombinant human CD3ζ protein is produced using expression systems like CHO or E. coli, often tagged for purification. Its study has advanced immunotherapy development, particularly in chimeric antigen receptor (CAR) T-cell therapies, where the CD3ζ domain is fused with antigen-binding domains to redirect T-cell specificity against cancer cells. Additionally, recombinant CD3ζ is used to investigate TCR signaling dysregulation in autoimmune diseases and immune deficiencies. Its role in modulating T-cell exhaustion and anergy also makes it a focus in chronic infection and cancer immunotherapy research. This protein’s functional versatility underscores its importance in both basic immunology and therapeutic innovation.
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