| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD248 |
| Uniprot No | Q9HCU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-757aa |
| 氨基酸序列 | MLLRLLLAWAAAGPTLGQDPWAAEPRAACGPSSCYALFPRRRTFLEAWRACRELGGDLATPRTPEEAQRVDSLVGAGPASRLLWIGLQRQARQCQLQRPLRGFTWTTGDQDTAFTNWAQPASGGPCPAQRCVALEASGEHRWLEGSCTLAVDGYLCQFGFEGACPALQDEAGQAGPAVYTTPFHLVSTEFEWLPFGSVAAVQCQAGRGASLLCVKQPEGGVGWSRAGPLCLGTGCSPDNGGCEHECVEEVDGHVSCRCTEGFRLAADGRSCEDPCAQAPCEQQCEPGGPQGYSCHCRLGFRPAEDDPHRCVDTDECQIAGVCQQMCVNYVGGFECYCSEGHELEADGISCSPAGAMGAQASQDLGDELLDDGEDEEDEDEAWKAFNGGWTEMPGILWMEPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATRPTLPSAHQPPVIPATHPALSRDHQIPVIAANYPDLPSAYQPGILSVSHSAQPPAHQPPMISTKYPELFPAHQSPMFPDTRVAGTQTTTHLPGIPPNHAPLVTTLGAQLPPQAPDALVLRTQATQLPIIPTAQPSLTTTSRSPVSPAHQISVPAATQPAALPTLLPSQSPTNQTSPISPTHPHSKAPQIPREDGPSPKLALWLPSPAPTAAPTALGEAGLAEHSQRDDRWLLVALLVPTCVFLVVLLALGIVYCTRCGPHAPNKRITDCYRWVIHAGSKSPTEPMPPRGSLTGVQTCRTSV |
| 分子量 | 107.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and Functional Characterization of Human CD248 as a Therapeutic Target in Tumor Angiogenesis"**
- **作者**: Simon A. Mortimer 等
- **摘要**: 该研究通过X射线晶体学解析了CD248的胞外结构域，揭示了其与配体相互作用的机制，并证明其重组蛋白在抑制肿瘤血管生成中的潜力，为癌症治疗提供了新靶点。
2. **"CD248/Endosialin: A Stromal Marker in Tumor Microenvironment Promotes Metastasis via Integrin Signaling"**
- **作者**: Kristian Pietras 等
- **摘要**: 文献阐明了CD248在肿瘤间质细胞中的高表达，通过整合素信号通路促进肿瘤转移和血管重塑，强调了其作为预后标志物和治疗靶点的价值。
3. **"CD248 Regulates Extracellular Matrix Remodeling in Fibrotic Diseases"**
- **作者**: Raghu Kalluri 团队
- **摘要**: 研究证明CD248在纤维化组织中通过调控胶原沉积和成纤维细胞活化驱动组织硬化，靶向CD248可改善肝/肺纤维化模型中的病理进程。
4. **"Endothelial CD248 Modulates Vascular Inflammation and Atherosclerosis"**
- **作者**: Catherine M. Shanahan 等
- **摘要**: 该研究揭示了CD248在内皮细胞炎症反应中的调控作用，发现其缺失可减少动脉粥样硬化斑块形成，提示其在心血管疾病中的治疗意义。
(注：以上为示例性内容，实际文献需根据PubMed/Google Scholar等数据库检索具体标题及作者。)
**Background of Recombinant Human Endosialin (CD248)**
CD248. also known as endosialin or TEM1. is a transmembrane glycoprotein belonging to the C-type lectin-like protein family. Primarily expressed on vascular endothelial cells, pericytes, and stromal fibroblasts during tissue development or remodeling, it is markedly upregulated in pathological conditions such as tumor angiogenesis, fibrosis, and chronic inflammation. CD248 interacts with extracellular matrix (ECM) components like fibronectin and collagen, modulating cell adhesion, migration, and signaling through its cytoplasmic domain.
Recombinant human CD248 is engineered to study its structure-function relationships and therapeutic potential. It typically retains key domains, including an N-terminal lectin-like domain, sushi repeats, and a mucin-rich region, critical for ligand binding and cellular communication. As a biomarker, CD248 is implicated in tumor progression by promoting ECM remodeling and stromal-tumor crosstalk, making it a target for anticancer therapies.
Current research focuses on neutralizing antibodies, small-molecule inhibitors, and CAR-T cell strategies to block CD248 activity. Its role in fibrotic diseases and wound healing is also under exploration, highlighting its dual implications in both health and disease.
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