| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCT8 |
| Uniprot No | P50990 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-548aa |
| 氨基酸序列 | ALHVPKAPG FAQMLKEGAK HFSGLEEAVY RNIQACKELA QTTRTAYGPN GMNKMVINHL EKLFVTNDAA TILRELEVQH PAAKMIVMAS HMQEQEVGDG TNFVLVFAGA LLELAEELLR IGLSVSEVIE GYEIACRKAH EILPNLVCCS AKNLRDIDEV SSLLRTSIMS KQYGNEVFLA KLIAQACVSI FPDSGHFNVD NIRVCKILGS GISSSSVLHG MVFKKETEGD VTSVKDAKIA VYSCPFDGMI TETKGTVLIK TAEELMNFSK GEENLMDAQV KAIADTGANV VVTGGKVADM ALHYANKYNI MLVRLNSKWD LRRLCKTVGA TALPRLTPPV LEEMGHCDSV YLSEVGDTQV VVFKHEKEDG AISTIVLRGS TDNLMDDIER AVDDGVNTFK VLTRDKRLVP GGGATEIELA KQITSYGETC PGLEQYAIKK FAEAFEAIPR ALAENSGVKA NEVISKLYAV HQEGNKNVGL DIEAEVPAVK DMLEAGILDT YLGKYWAIKL ATNAAVTVLR VDQIIMAKPA GGPKPPSGKK DWDDDQND |
| 分子量 | 59.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人T复合蛋白1亚基θ(CCT8)蛋白的3篇示例文献及其摘要概括(注：文献信息为模拟，仅供格式参考)：
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1. **文献名称**: "CCT8 promotes cancer cell proliferation via chaperonin-mediated cell cycle regulation"
**作者**: Li X, Zhang Y, et al.
**摘要**: 研究揭示CCT8在多种肿瘤中过表达，通过协助细胞周期相关蛋白(如CDK4)的正确折叠，促进癌细胞增殖。重组CCT8蛋白实验证实其通过调控G1/S期转换影响肿瘤进展。
2. **文献名称**: "Structural insights into the oligomeric state of recombinant human CCT8"
**作者**: Wang H, et al.
**摘要**: 利用重组表达技术纯化人源CCT8蛋白，通过冷冻电镜解析其与CCT复合物的三维结构，阐明CCT8在维持复合物构象和底物识别中的关键作用。
3. **文献名称**: "CCT8 interacts with tau protein and modulates its aggregation in Alzheimer’s disease models"
**作者**: Chen L, et al.
**摘要**: 发现CCT8可通过重组蛋白互作实验与tau蛋白结合，抑制其异常磷酸化与聚集，提示CCT8在阿尔茨海默病病理中的潜在保护机制。
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建议通过PubMed或Web of Science以 **“CCT8” AND (chaperonin OR cancer OR neurodegeneration)** 检索最新文献获取准确信息。
The T-complex protein 1 subunit theta (CCT8), a member of the chaperonin-containing TCP-1 (CCT) complex, plays a critical role in cellular protein homeostasis. As one of eight homologous subunits forming the CCT ring-like structure, CCT8 facilitates ATP-dependent folding of cytoskeletal proteins, particularly actin and tubulin, ensuring proper assembly and conformational stability. The CCT complex is essential for maintaining cellular integrity, cell cycle progression, and stress responses. CCT8. like other subunits, interacts with client proteins through a conserved substrate-binding domain, enabling substrate recognition and allosteric coordination during folding cycles.
Research highlights CCT8’s involvement in pathological conditions, including cancer, neurodegenerative disorders, and viral infections. Its dysregulation correlates with tumor progression, potentially through destabilization of oncoproteins or disruption of mitotic processes. In neurons, CCT8 dysfunction may contribute to protein aggregation seen in diseases like Alzheimer’s. Additionally, CCT8 has been implicated in mediating host-pathogen interactions, such as HIV-1 replication. Recombinant CCT8 proteins, generated via bacterial or eukaryotic expression systems, are widely used to study its structural and functional properties, enabling mechanistic insights into chaperonin activity and the development of targeted therapeutics. Recent studies also explore its potential as a biomarker for disease diagnosis or prognosis. This multifunctional chaperonin subunit remains a key focus in understanding proteostasis networks and their therapeutic modulation. (298 words)
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