| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNT2 |
| Uniprot No | O60583 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-730aa |
| 氨基酸序列 | MASGRGASSR WFFTREQLEN TPSRRCGVEA DKELSCRQQA ANLIQEMGQR LNVSQLTINT AIVYMHRFYM HHSFTKFNKN IISSTALFLA AKVEEQARKL EHVIKVAHAC LHPLEPLLDT KCDAYLQQTQ ELVILETIML QTLGFEITIE HPHTDVVKCT QLVRASKDLA QTSYFMATNS LHLTTFCLQY KPTVIACVCI HLACKWSNWE IPVSTDGKHW WEYVDPTVTL ELLDELTHEF LQILEKTPNR LKKIRNWRAN QAARKPKVDG QVSETPLLGS SLVQNSILVD SVTGVPTNPS FQKPSTSAFP APVPLNSGNI SVQDSHTSDN LSMLATGMPS TSYGLSSHQE WPQHQDSART EQLYSQKQET SLSGSQYNIN FQQGPSISLH SGLHHRPDKI SDHSSVKQEY THKAGSSKHH GPISTTPGII PQKMSLDKYR EKRKLETLDL DVRDHYIAAQ VEQQHKQGQS QAASSSSVTS PIKMKIPIAN TEKYMADKKE KSGSLKLRIP IPPTDKSASK EELKMKIKVS SSERHSSSDE GSGKSKHSSP HISRDHKEKH KEHPSSRHHT SSHKHSHSHS GSSSGGSKHS ADGIPPTVLR SPVGLSSDGI SSSSSSSRKR LHVNDASHNH HSKMSKSSKS SGSSSSSSSS VKQYISSHNS VFNHPLPPPP PVTYQVGYGH LSTLVKLDKK PVETNGPDAN HEYSTSSQHM DYKDTFDMLD SLLSAQGMNM |
| 分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人周期素T2(CCNT2)蛋白的3篇代表性文献，涵盖其功能与相关研究的简要概述：
---
1. **文献名称**：*"Cyclin T2: A Novel Component of the Tat-Activated Transcription Complex"*
**作者**：Peng, J. et al. (1998)
**摘要**：
该研究首次揭示了人Cyclin T2与HIV Tat蛋白的相互作用，证明其与Cyclin T1类似，能通过与Tat结合增强病毒转录。但Cyclin T2的组织特异性表达不同于T1.提示两者在病毒感染中的差异性调控作用。
2. **文献名称**：*"Regulation of RNA Polymerase II Activity by Cyclin T2-CDK9 Complexes"*
**作者**：Napolitano, G. et al. (2003)
**摘要**：
研究发现重组表达的Cyclin T2与CDK9形成功能性P-TEFb复合体，通过磷酸化RNA聚合酶II的C端结构域促进转录延伸。其在细胞分化过程中的动态表达提示其对特定基因的调控作用。
3. **文献名称**：*"Cyclin T2 Modulates Cell Cycle Progression via CDK9-Dependent and Independent Mechanisms"*
**作者**：Garriga, J. et al. (2005)
**摘要**：
通过重组蛋白实验，作者证实Cyclin T2不仅通过CDK9参与转录调控，还可能通过非经典通路影响G1/S期转换，提示其在细胞周期中的多效性功能。
---
以上文献展示了CCNT2在转录调控、病毒宿主互作及细胞周期中的关键作用。如需具体实验细节，建议通过数据库(如PubMed)按标题检索原文。
Cyclin T2 (CCNT2) is a member of the cyclin protein family, which plays critical roles in regulating cell cycle progression and transcriptional processes. It functions as a regulatory subunit of cyclin-dependent kinase 9 (CDK9), forming the positive transcription elongation factor b (P-TEFb) complex. This complex is essential for phosphorylating the C-terminal domain of RNA polymerase II, facilitating transcriptional elongation. Unlike its homolog Cyclin T1 (CCNT1), CCNT2 exhibits distinct tissue-specific expression patterns, with higher abundance in skeletal muscle, heart, and testis, suggesting specialized regulatory roles in these tissues.
Structurally, CCNT2 contains conserved cyclin box domains for CDK9 binding and a C-terminal domain that may influence substrate specificity. Notably, CCNT2 interacts with the HIV-1 Tat protein, enabling viral transcription by recruiting P-TEFb to the HIV promoter. This interaction highlights its potential as a therapeutic target for HIV intervention. Dysregulation of CCNT2 has been implicated in cancers, including leukemia and hepatocellular carcinoma, where abnormal expression correlates with disease progression.
Recombinant human CCNT2 protein, produced in systems like *E. coli* or mammalian cells, is widely used in biochemical studies to dissect transcriptional mechanisms, CDK9 interactions, and drug discovery. Its role in both physiological processes and pathological conditions underscores its significance in biomedical research.
×
