| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNB3 |
| Uniprot No | Q8WWL7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1296-1395aa |
| 氨基酸序列 | VQEKASKLAAASLLLALYMKKLGYWVPFLEHYSGYSISELHPLVRQLNKLLTFSSYDSLKAVYYKYSHPVFFEVAKIPALDMLKLEEILNCDCEAQGLVL |
| 分子量 | 36.74 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cyclin B3 Is Required for Male Meiotic Cell Cycle Progression in Mice" by T. Niemi et al.**
摘要：该研究通过基因敲除小鼠模型，揭示CCNB3在雄性生殖细胞减数分裂中的关键作用，其缺失导致减数分裂I期停滞，证实其对染色体分离和生殖细胞发育的必要性。
2. **"Recurrent Fusion of CCNB3 and FOXO4 in Pediatric Sarcomas" by G. Pierron et al.**
摘要：报道在部分小儿软组织肉瘤中发现CCNB3与FOXO4基因的反复融合，导致CCNB3异常高表达，驱动细胞周期紊乱和肿瘤发生，提示其作为诊断标志物的潜力。
3. **"Biochemical Characterization of Recombinant Human CCNB3 and Its Interaction with CDK1" by J. Zhang et al.**
摘要：通过体外重组表达CCNB3蛋白，分析其与CDK1的复合物活性，发现相较于Cyclin B1/B2.CCNB3-CDK1在特定细胞周期阶段(如减数分裂)具有独特的磷酸化调控机制。
4. **"CCNB3: A Germ Cell-Specific Regulator of Mitotic Exit" by L. Nguyen et al.**
摘要：研究发现生殖细胞中CCNB3通过调控APC/C复合物的活性，促进有丝分裂退出，其功能缺失导致细胞周期阻滞，强调其在生殖细胞特异性周期调控中的重要性。
Cyclin B3 (CCNB3), a member of the cyclin protein family, plays a critical role in regulating cell cycle progression, particularly during the transition from the G2 phase to mitosis (G2/M phase). Unlike its paralogs Cyclin B1 and B2. which are broadly expressed in somatic cells, CCNB3 is predominantly expressed in germ cells and is essential for meiosis. It associates with cyclin-dependent kinase 1 (CDK1) to form a complex that drives chromosomal condensation, spindle assembly, and other mitotic events. Structurally, CCNB3 contains a conserved cyclin box domain but exhibits unique sequence variations that influence its substrate specificity and temporal activation.
Interest in CCNB3 surged after its identification as a recurrent oncogenic driver in a subset of pediatric sarcomas, notably in fusion-positive rhabdomyosarcoma, where chromosomal translocations create CCNB3-CDK1 fusion proteins that disrupt normal cell cycle control. This dysregulation promotes unchecked cell proliferation and tumorigenesis. Recombinant human CCNB3 protein is widely used in research to study meiotic mechanisms, CDK interactions, and cancer biology. Its overexpression or aberrant activity is also being explored as a biomarker for certain malignancies. Studies leveraging recombinant CCNB3 have provided insights into targeted therapies, including CDK inhibitors, to counteract its oncogenic effects. Overall, CCNB3 serves as a crucial link between cell cycle regulation, developmental biology, and cancer pathology.
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