| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | Ccl27a |
| Uniprot No | Q9Z1X0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-120aa |
| 氨基酸序列 | LPLPSSTSCCTQLYRQPLPSRLLRRIVHMELQEADGDCHLQAVVLHLARRSVCVHPQNRSLARWLERQGKRLQGTVPSLNLVLQKKMYSNPQQQN |
| 分子量 | 10.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组小鼠CCL27a蛋白的参考文献示例(文献信息为模拟构造，仅供参考)：
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1. **文献名称**: *"Characterization of Recombinant Murine CCL27a and Its Role in Skin-Specific T Cell Homing"*
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌系统中表达了重组小鼠CCL27a蛋白，证实其能有效趋化皮肤归巢记忆T细胞，并通过体外迁移实验证明其与CCR10受体的特异性结合，为皮肤免疫研究提供了工具。
2. **文献名称**: *"Functional Analysis of Ccl27a in a Murine Psoriasis Model"*
**作者**: Tanaka K, et al.
**摘要**: 通过重组小鼠CCL27a蛋白局部注射，发现其加重了咪喹莫特诱导的银屑病样皮炎，提示其在皮肤炎症中具有促炎作用，可能与Th17细胞募集相关。
3. **文献名称**: *"Expression and Purification of Bioactive Recombinant CCL27a in a Mammalian System"*
**作者**: Lee S, et al.
**摘要**: 利用HEK293细胞成功分泌具有生物活性的重组小鼠CCL27a，经质谱验证结构正确，功能实验显示其诱导角质形成细胞产生IL-8.支持其在表皮屏障调控中的潜在作用。
4. **文献名称**: *"CCL27a-CCR10 Axis Regulates Langerhans Cell Migration in Contact Hypersensitivity"*
**作者**: Müller G, et al.
**摘要**: 研究使用重组小鼠CCL27a蛋白证明其可增强Langerhans细胞向淋巴结的迁移，依赖于CCR10信号通路，为接触性超敏反应的机制提供了新见解。
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(注：上述文献为示例性质，实际引用时需查询真实数据库验证。)
Ccl27a, also known as cutaneous T-cell-attracting chemokine (CTACK), is a small chemokine protein primarily expressed in murine epidermal keratinocytes. It plays a crucial role in skin-specific immune responses by recruiting CCR10-expressing T lymphocytes to cutaneous sites. This protein is constitutively produced under homeostatic conditions and upregulated during inflammatory processes, functioning as a key mediator in cutaneous inflammation and immune surveillance.
As a member of the CC chemokine family, Ccl27a features a conserved structural motif with two adjacent cysteine residues. The recombinant murine Ccl27a protein is typically generated through genetic engineering in expression systems like E. coli or mammalian cells, enabling researchers to study its biological activity in vitro and in vivo. Recombinant forms maintain the protein's functional capacity to bind and activate the CCR10 receptor, facilitating investigations into T-cell migration patterns and cutaneous immunity mechanisms.
Studies using recombinant Ccl27a have revealed its involvement in various dermatological conditions, including atopic dermatitis, psoriasis, and cutaneous lymphomas. Its role extends to maintaining skin barrier integrity and mediating responses to UV radiation exposure. Current research focuses on leveraging recombinant Ccl27a as a therapeutic target or biomarker for inflammatory skin diseases, while also exploring its potential in vaccine development and immunotherapy strategies through controlled lymphocyte trafficking modulation.
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