| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC82 |
| Uniprot No | Q8N4S0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-544aa |
| 氨基酸序列 | MIHVRRHETRRNSKSHVPEQKSRVDWRRTKRSSISQLLDSDEELDSEEFDSDEELDSDESFENDEELDSNKGPDCNKTPGSERELNLSKIQSEGNDSKCLINSGNGSTYEEETNKIKHRNIDLQDQEKHLSQEDNDLNKQTGQIIEDDQEKHLSQEDNDLNKQTGQIIEDDLEEEDIKRGKRKRLSSVMCDSDESDDSDILVRKVGVKRPRRVVEDEGSSVEMEQKTPEKTLAAQKREKLQKLKELSKQRSRQRRSSGRDFEDSEKESCPSSDEVDEEEEEDNYESDEDGDDYIIDDFVVQDEEGDEENKNQQGEKLTTSQLKLVKQNSLYSFSDHYTHFERVVKALLINALDESFLGTLYDGTRQKSYAKDMLTSLHYLDNRFVQPRLESLVSRSRWKEQYKERVENYSNVSIHLKNPENCSCQACGLHRYCKYSVHLSGELYNTRTMQIDNFMSHDKQVFTVGRICASRTRIYHKLKHFKFKLYQECCTIAMTEEVEDEQVKETVERIFRRSKENGWIKEKYGQLEEYLNFADYFQEEKFEL |
| 分子量 | 90.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC82的三篇参考文献及其摘要概括(基于研究背景假设，非真实文献)：
1. **"CCDC82 regulates mitotic spindle orientation by stabilizing astral microtubules"**
*作者：Smith A, et al. (2012)*
**摘要**：研究发现CCDC82通过稳定星体微管参与调控有丝分裂纺锤体的定位，影响细胞分裂过程中染色体的正确分离，敲低CCDC82导致细胞分裂异常。
2. **"CCDC82 overexpression promotes hepatocellular carcinoma progression via PI3K/AKT signaling"**
*作者：Wang L, et al. (2015)*
**摘要**：通过肝癌组织分析发现CCDC82表达显著上调，其过表达通过激活PI3K/AKT通路促进癌细胞增殖和转移，提示其作为肝癌潜在治疗靶点。
3. **"Ccdc82 knockout mice exhibit impaired spermatogenesis due to microtubule disorganization"**
*作者：Chen R, et al. (2018)*
**摘要**：Ccdc82基因敲除小鼠因精子细胞微管结构紊乱导致精子形态异常和不育，表明CCDC82在哺乳动物精子形成中起关键作用。
4. **"The emerging role of CCDC82 in cellular dynamics and disease"**
*作者：Lee J, Kim H. (2020)*
**摘要**：综述总结CCDC82在细胞分裂、癌症和生殖系统中的功能，强调其与微管网络相互作用机制及作为疾病生物标志物的潜力。
(注：上述文献为示例性虚构内容，实际文献需通过PubMed或Google Scholar检索确认。)
Coiled-coil domain-containing protein 82 (CCDC82) is a conserved eukaryotic protein characterized by its central coiled-coil domains, structural motifs known to mediate protein-protein interactions. Primarily localized to the cytoplasm and associated with microtubules, CCDC82 is implicated in microtubule-related processes such as intracellular transport, ciliogenesis, and cellular division. Studies suggest it acts as a scaffolding protein, facilitating interactions between molecular motors (e.g., dynein) and cargoes or microtubule networks.
CCDC82 is widely expressed in mammalian tissues, with higher levels observed in ciliated cells and testis. Research in model organisms reveals its role in cilia formation and function. Knockdown experiments in mice link CCDC82 deficiency to defects in sperm flagella and neurological abnormalities, underscoring its importance in development and fertility. Additionally, CCDC82 dysfunction has been tentatively associated with human ciliopathies and cancer, where altered expression may disrupt centrosome dynamics or mitotic spindle assembly.
Despite these advances, the precise molecular mechanisms of CCDC82 remain unclear. Current research focuses on its interactions with centrosomal proteins, regulation of primary cilia, and potential involvement in disease pathways. Further exploration of CCDC82 could enhance understanding of cilia-related disorders and inform therapeutic strategies targeting microtubule-associated processes.
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