| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC78 |
| Uniprot No | A2IDD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-438aa |
| 氨基酸序列 | MEHAATTGPR PGPPSRRVEN VVLRAKDWLP GAPGGTAVWA TSLEAEVPPD LALNKEQQLQ ISKELVDIQI TTHHLHEQHE AEIFQLKSEI LRLESRVLEL ELRGDGTSQG CAVPVESDPR HPRAAAQELR HKAQVPGHSD DHRFQVQPKN TMNPENEQHR LGSGLQGEVK WALEHQEARQ QALVTRVATL GRQLQGAREE ARAAGQRLAT QAVVLCSCQG QLRQAEAENA RLQLQLKKLK DEYVLRLQHC AWQAVEHADG AGQAPATTAL RTFLEATLED IRAAHRSREQ QLARAARSYH KRLVDLSRRH EELLVAYRAP GNPQAIFDIA SLDLEPLPVP LVTDFSHRED QHGGPGALLS SPKKRPGGAS QGGTSEPQGL DAASWAQIHQ KLRDFSRSTQ SWNGSGHSCW SGPRWLKSNF LSYRSTWTST WAGTSTKS |
| 分子量 | 74.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于CCDC78研究的模拟参考文献示例(非真实文献)：
1. **文献名称**: *CCDC78 interacts with microtubule-associated proteins to regulate ciliary assembly*
**作者**: Watanabe Y, Höög C.
**摘要**: 研究揭示了CCDC78通过与微管结合蛋白(如TUBG1)相互作用，调控纤毛结构的形成和稳定性，在体外模型中敲除CCDC78导致纤毛长度异常和细胞运动缺陷。
2. **文献名称**: *The role of CCDC78 in spermatogenesis: a knockout mouse study*
**作者**: Li X, et al.
**摘要**: 通过构建CCDC78基因敲除小鼠模型，发现其精子发生过程中出现鞭毛结构异常，表明CCDC78对精子尾部发育及男性生育能力至关重要。
3. **文献名称**: *CCDC78 modulates centrosome duplication via binding to CEP192*
**作者**: Park JH, et al.
**摘要**: 该研究证实CCDC78与中心体蛋白CEP192结合，抑制过度复制，维持细胞有丝分裂的正常进程，CCDC78缺失可能引发染色体不稳定性和肿瘤发生。
4. **文献名称**: *CCDC78 as a potential biomarker in lung adenocarcinoma*
**作者**: Chen L, et al.
**摘要**: 通过癌症基因组数据分析，发现CCDC78在肺腺癌中高表达，并与患者预后不良相关，可能通过激活MAPK信号通路促进肿瘤侵袭。
(注：以上文献标题及内容为学术场景模拟，实际研究请参考真实数据库。)
Coiled-coil domain-containing protein 78 (CCDC78) is a human protein encoded by the *CCDC78* gene, primarily expressed in germ cells, neuronal tissues, and certain cancer types. It belongs to the coiled-coil domain protein family, characterized by structural motifs that mediate protein-protein interactions, cytoskeletal organization, and intracellular trafficking. CCDC78 is noted for its localization to microtubule-associated structures, including the manchette in developing spermatids and the spindle apparatus during cell division. Its involvement in spermiogenesis has been highlighted, with studies suggesting a role in shaping the sperm head and regulating flagellar development through interactions with microtubules and cytoskeletal regulators.
In cancer, *CCDC78* mRNA overexpression has been observed in testicular germ cell tumors, though its functional significance remains unclear. Emerging evidence also links CCDC78 to neurological processes, potentially influencing synaptic plasticity and ion channel activity. Despite these associations, the precise molecular mechanisms and signaling pathways regulated by CCDC78 are poorly understood. Current research focuses on its structural domains, interactome profiling, and potential as a biomarker or therapeutic target in fertility disorders or cancers. Further studies are needed to unravel its physiological and pathological roles, particularly its dual functions in cellular dynamics and disease contexts.
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