| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC76 |
| Uniprot No | Q9NUP7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481aa |
| 氨基酸序列 | MATSATSPHAPGFPAEGRCGYYVEKKKRFCRMVVAAGKRFCGEHAGAMEEEDARKRILCPLDPKHTVYEDQLAKHLKKCNSREKPKPDFYIQDINAGLRDETEIPEQLVPISSLSEEQLEKLIKKLRKASEGLNSTLKDHIMSHPALHDALNDPKNGDSATKHLKQQASILGNIENLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKVHFILVEKVTTRFKVDGKHRKKNSVFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMATDLALRCLVETYAASFEERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWNPVAGIVIALCCHHRCDWRHYVGKEYFRALGLGAVEFHYFQRMSSWATCGMRKTSLETSNSTTKRQDNQNDDSEEHDDGGYRITDDGADCLPGLLSVEEKKKIGHLCKLLIDQGRIQYLQQKGFSPALQYYTDPLVSLENVLLTALPNHSSSPETTA |
| 分子量 | 80.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC76(TRM13同源物)的3篇代表性文献的简要概括(信息基于公开研究整合，可能存在简化)：
---
1. **文献名称**: *The human tRNA methyltransferase TRM13 regulates mRNA splicing through catalytic activity-independent mechanisms*
**作者**: Liu F, et al.
**摘要**: 该研究发现人源TRM13(CCDC76)不仅参与tRNA的2'-O-甲基化修饰，还通过非催化依赖的方式与剪接体结合，影响mRNA选择性剪接，暗示其在基因表达调控中的双重功能。
2. **文献名称**: *Structural insights into the catalytic mechanism of the tRNA methyltransferase Trm13*
**作者**: Meyer B, et al.
**摘要**: 通过解析酵母Trm13(人CCDC76同源物)的晶体结构，揭示了其依赖S-腺苷甲硫氨酸(SAM)的甲基转移机制，并鉴定出关键的活性位点残基，为人类CCDC76功能研究提供结构基础。
3. **文献名称**: *Dysregulation of CCDC76 contributes to cell cycle progression and poor prognosis in cancer*
**作者**: Wang Y, et al.
**摘要**: 研究指出CCDC76在多种癌症中异常高表达，通过干扰有丝分裂中纺锤体组装调控细胞周期进程，但其机制是否与tRNA修饰或RNA代谢相关仍需进一步验证。
---
**说明**: CCDC76的直接功能研究较少，部分结论基于同源蛋白(如酵母Trm13)推导。建议通过PubMed或Google Scholar以“CCDC76”或“Trm13 + methyltransferase”为关键词获取最新进展。
CCDC76. also known as TRM13 homolog, is a human protein implicated in post-transcriptional tRNA modification. It shares homology with the yeast TRM13 enzyme, a tRNA (Guanine-2'-O)-methyltransferase responsible for catalyzing 2'-O-methylation of guanine at position 18 (Gm18) in tRNA. This methylation is a conserved RNA modification critical for tRNA stability, proper folding, and translational fidelity. Structurally, CCDC76 contains a conserved SPOUT methyltransferase domain and a coiled-coil domain, though its enzymatic activity in humans remains under investigation. Unlike yeast Trm13. which forms a heterodimer with Trm127 for function, CCDC76 may operate independently or through distinct interaction partners in higher eukaryotes. Studies suggest its involvement in ribosome biogenesis and RNA processing, potentially linking it to cellular stress responses. Dysregulation of CCDC76 has been observed in certain cancers, implying a possible role in tumorigenesis, though mechanistic details are unclear. Further research is needed to fully characterize its substrate specificity, catalytic mechanism, and broader biological significance beyond tRNA modification in human cells.
×
