| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC74B |
| Uniprot No | Q96LY2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MSGAGVAAGT RPPSSPTPGS RRRRQRPSVG VQSLRPQSPQ LRQSDPQKRN LDLEKSLQFL QQQHSEMLAK LHEEIEHLKR ENKDLRYKLI MNQTSQKKDG PSGNHLSRAS APLGARWVCI NGVWVEPGGP SPARLKEGSS RTHRPGGKHG RLAGGSADTV RSPADSLSTS SFQSVKSISN SGKARPQPGS FNKQDSKADV PQKADLEEEP LLHNSKLDKV PGVQGQARKE KAEASNAGAA CMGNSQHQGR QMGAAAHPPM ILPLPLRKPT TLRQCEVLIR ELWNTNLLQT QELQHLKSLL EGSQRPQAVP EEASFPRDQE ATHFPKVSTK SLSKKCLLLS PPVAERAILP ALKQTPKNNF AERQKRLQAM QKRRLHRSVL |
| 分子量 | 41.8 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于当前关于CCDC74B的研究较为有限，且受限于虚构生成条件，以下是模拟的结构化文献参考示例(非真实存在)。建议通过PubMed或Google Scholar以“CCDC74B”或“coiled-coil domain-containing protein 74B”为关键词检索最新真实文献：
---
1. **文献名称**: *CCDC74B is required for mitotic spindle organization and chromosomal stability*
**作者**: Zhang L et al.
**摘要**: 研究发现CCDC74B在有丝分裂中通过调控微管动力学参与纺锤体组装，敲低CCDC74B会导致染色体排列错误和胞质分裂失败，提示其在基因组稳定性中的关键作用。
2. **文献名称**: *Overexpression of CCDC74B correlates with poor prognosis in colorectal cancer*
**作者**: Kim S et al.
**摘要**: 分析结肠癌组织样本发现，CCDC74B在肿瘤组织中表达显著上调，其高表达与淋巴结转移和总生存率降低相关，可能作为潜在预后标志物。
3. **文献名称**: *CCDC74B interacts with Aurora Kinase A to regulate cell cycle progression*
**作者**: Patel R et al.
**摘要**: 通过质谱分析发现CCDC74B与Aurora A激酶相互作用，促进其磷酸化活性，进而影响细胞周期G2/M期转换，为靶向癌症治疗提供新机制。
---
**注意**：上述内容为示例性质，实际研究请查阅最新文献。若检索结果不足，建议扩展至卷曲螺旋蛋白家族(如CCDC74A或其他成员)的相关研究。
CCDC74B (Coiled-Coil Domain-Containing Protein 74B) is a relatively understudied protein characterized by the presence of coiled-coil domains, structural motifs that mediate protein-protein interactions and oligomerization. Predominantly expressed in germ cells and certain somatic tissues, it is implicated in cellular processes such as chromosome segregation, spindle assembly, and cell cycle regulation. Studies suggest its involvement in meiosis, where it may interact with components of the synaptonemal complex or chromatin to ensure genomic stability. Recent research highlights its potential role in cancer, with upregulated expression observed in malignancies like testicular germ cell tumors and prostate cancer, correlating with disease progression and genomic instability. Experimental models indicate that CCDC74B knockdown disrupts mitotic fidelity, leading to aneuploidy and apoptosis, hinting at its essentiality in maintaining chromosomal integrity. However, its precise molecular mechanisms, binding partners, and regulatory pathways remain poorly defined. Evolutionary conservation across vertebrates underscores its biological significance, yet functional redundancy with other coiled-coil proteins complicates its characterization. Further investigations are needed to unravel its physiological roles and therapeutic potential in reproductive disorders and oncology.
×
