| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC57 |
| Uniprot No | Q2TAC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-751aa |
| 氨基酸序列 | MLPLGSEPALNELLLRKEEEWRALQAHRTQLQEAALQDTRSQLEEAQGKLRCLQEDFVYNLQVLEERDLELERYDAAFAQAREWEEARRAEVSELKIEAAKLRQALAREARKVEELQQQQQLAFQEHRLELERVHSDKNGEIDHHREQYENLKWTLERKLEELDGELALQRQELLLEFESKMRKREHEFRLQADNMSNTALSRELKVKLLHKELEALKEAGAKAAESLQRAEATNAELERKLQSRAGELQDLEAMSRARVKDLEDKLHSVQLTRKKEEETFKRKHEELDRLAREKDAVLVAVKGAHVEQLQELQTRVLELQAHCETLEAQLRRAEWRQADTAKEKDAAIDQLREDASTVKSAWDAQIAQLSKEMVSRDLQIQTLQEEEVKLKAQVARSQQDIERYKQQLSLAVERERSLERDQVQLGLDWQRRCDDIERDQIQKSEALIQGLSMAKSQVAAKLQETEQALQEQEVVLKAMTLERDQAVQALRMHGLPRPGAQMLLRQHEEEISKDFPSSEIQRLREQNTSLRNAIAQMRKEMEALSHQIPPPIQTAAESTDANQPDPEAGGDAATPDYVLALEAEIRTLKHKFKTLEKHLEDVLDPLKMSSPHAESQPSVRTSTETTGGSAQAGQAGGSVQAGQAGGSVQAGPVSSGLALRKLGDRVQLLNLLVTRLRQKVLREPLEPAALQRELPREVDQVHLEVLELRKQVAELGKHLRIAQHGGAEPSGRKQPPASDAVALGREVGAR |
| 分子量 | 111.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人卷曲螺旋结构域含57号蛋白(CCDC57)的模拟参考文献示例，内容基于科学研究的常见方向构建，供参考：
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1. **文献名称**：*"CCDC57 is Required for Mitotic Spindle Organization and Chromosome Segregation"*
**作者**：Zhang L, et al.
**摘要**：本研究揭示CCDC57通过其卷曲螺旋结构域与微管结合，调控有丝分裂纺锤体的组装和稳定性。敲低CCDC57导致染色体分离错误，提示其在细胞周期调控中的关键作用。
2. **文献名称**：*"Structural Insights into the Coiled-Coil Domains of CCDC57 and Interaction with Centrosomal Proteins"*
**作者**：Thompson RK, et al.
**摘要**：通过冷冻电镜解析CCDC57的卷曲螺旋结构域，发现其形成二聚体并与中心体蛋白CEP135结合，可能参与中心体成熟和纤毛发生的过程。
3. **文献名称**：*"CCDC57 Dysfunction Impairs Primary Cilia Formation and Promotes Renal Cystogenesis"*
**作者**：Guo Y, et al.
**摘要**：在小鼠模型中，CCDC57缺失导致肾上皮细胞纤毛形成缺陷，并诱发多囊肾病，表明其通过调控纤毛相关信号通路维持组织稳态。
4. **文献名称**：*"CCDC57 Acts as a Tumor Suppressor by Modulating MTOR Signaling in Hepatocellular Carcinoma"*
**作者**：Chen H, et al.
**摘要**：CCDC57在肝癌中表达下调，过表达CCDC57抑制癌细胞增殖和迁移，机制涉及与mTOR通路蛋白互作并调控自噬过程。
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**说明**：以上文献为示例性内容，实际研究需查阅权威数据库(如PubMed、Web of Science)获取。建议使用关键词“CCDC57”或“coiled-coil domain containing protein 57”结合研究方向进行检索。
Coiled-coil domain-containing protein 57 (CCDC57) is a conserved eukaryotic protein characterized by multiple coiled-coil motifs, which mediate protein-protein interactions and structural scaffolding. Predominantly localized to the centrosome and microtubule-associated compartments, CCDC57 plays critical roles in centrosome function, microtubule organization, and cilium assembly. Studies suggest its involvement in cell cycle progression, mitotic spindle dynamics, and intracellular transport. CCDC57 interacts with key centrosomal components (e.g., CEP63) and is implicated in regulating centriole duplication and primary cilia formation. Dysregulation of CCDC57 has been linked to ciliopathies, reproductive disorders, and cancers, as its absence disrupts microtubule-dependent processes essential for development and tissue homeostasis. Knockout murine models exhibit male infertility due to impaired spermatogenesis, underscoring its role in germ cell differentiation. Additionally, CCDC57 is expressed in neural tissues, hinting at potential contributions to neurodevelopment. Despite progress, its exact molecular mechanisms remain partially unresolved. Recent proteomic studies highlight its association with the nuclear envelope and mitotic machinery, suggesting broader roles in nuclear-cytoplasmic coordination. Research continues to explore its post-translational modifications and tissue-specific isoforms. As a multifunctional scaffold protein, CCDC57 exemplifies the complexity of coiled-coil domain proteins in cellular architecture and signaling, warranting further investigation into its pathophysiological relevance.
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