| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC49 |
| Uniprot No | Q9NXE8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-425aa |
| 氨基酸序列 | MGGGDLNLKKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEEKLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEKEAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKKKKKEKKKKHKKHKHRSSSSDRSSSEDEHSAGRSQKKMANSSPVLSKVPGYGLQVRNSDRNQGLQGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKSTREAGSRDRRSRSLGRRSRSPRPSKLHNSKVNRRETGQTRSPSPKKEVYQRRHAPGYTRKLSAEELERKRQEMMENAKWREEERLNILKRHAKDEEREQRLEKLDSRDGKFIHRMKLESASTSSLEDRVKRNIYSLQRTSVALEKNFMKR |
| 分子量 | 76 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CCDC49蛋白的3篇参考文献示例(注：文献为假设性示例，实际检索时请以数据库结果为准)：
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1. **文献名称**：*CCDC49 regulates cell cycle progression via interaction with PLK1 in non-small cell lung cancer*
**作者**：Li, X. et al.
**摘要**：本研究利用重组人CCDC49蛋白，揭示了其通过与PLK1激酶相互作用调控有丝分裂的分子机制，并发现CCDC49过表达促进肺癌细胞增殖和迁移。
2. **文献名称**：*Structural characterization of human CCDC49 and its role in DNA damage repair*
**作者**：Wang, Y. et al.
**摘要**：通过重组表达CCDC49蛋白并解析其晶体结构，研究者发现其卷曲螺旋结构域对BRCA1复合体的招募至关重要，暗示其在DNA损伤修复通路中的潜在功能。
3. **文献名称**：*CCDC49 deficiency disrupts spermatogenesis in mice by impairing meiotic recombination*
**作者**：Sato, K. et al.
**摘要**：利用重组CCDC49蛋白进行体外结合实验，证明其与减数分裂特异性蛋白MEIOC的相互作用，并揭示CCDC49缺失导致小鼠精子发生异常的具体机制。
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**说明**：
由于CCDC49的研究相对较少，以上示例基于相关领域常见研究方向假设。实际文献需通过PubMed、Web of Science等平台，以“CCDC49”或“Coiled-coil domain-containing protein 49”为关键词检索，并筛选涉及重组蛋白技术的文章。若公开数据库中结果不足，可扩展检索其相互作用蛋白或相关通路的研究。
The human CCDC49 (Coiled-Coil Domain-Containing Protein 49) is a protein encoded by the CCDC49 gene, located on chromosome 11. It is characterized by multiple coiled-coil domains, structural motifs known to mediate protein-protein interactions and subcellular organization. While its precise biological function remains under investigation, CCDC49 is hypothesized to play roles in cellular processes such as cytoskeletal dynamics, intracellular trafficking, or cilia-related functions, given the involvement of other coiled-coil proteins in these pathways.
Expression studies indicate that CCDC49 is widely distributed across tissues, with higher levels observed in organs like the kidneys and testes. Emerging evidence suggests potential links to ciliopathies or developmental disorders, though direct mechanistic data are limited. Recombinant human CCDC49 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), serves as a critical tool for functional studies, antibody development, and structural analyses. Its purification often involves affinity tags (e.g., His-tag) for enhanced stability and yield.
Current research focuses on elucidating its interactome, post-translational modifications, and involvement in disease contexts, such as cancer or ciliary dysfunction. Further studies are required to define its molecular mechanisms and therapeutic potential.
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