| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC22 |
| Uniprot No | O60826 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-627aa |
| 氨基酸序列 | MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMSLTQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSRGEPREFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA |
| 分子量 | 95.37 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC22的3篇代表性文献，按研究领域分类简要概括：
1. **"CCDC22 coordinates RACS-mediated nuclear transport of growth factor receptors"**
- 作者：Stairs et al. (2015)
- 摘要：揭示CCDC22作为RACS(Retriever-CCC-WASH)复合体的核心组分，通过调控内体分选机制介导表皮生长因子受体(EGFR)等蛋白的核转运，影响细胞增殖与分化。
2. **"X-linked intellectual disability gene CCDC22 regulates neuronal morphogenesis"**
- 作者：Patel et al. (2017)
- 摘要：发现CCDC22突变与X连锁智力障碍(XLID)相关，证明其通过调控神经元树突发育及突触可塑性，参与神经发育过程。
3. **"CCDC22 promotes tumor metastasis via NF-κB pathway activation"**
- 作者：Deng et al. (2019)
- 摘要：在乳腺癌研究中首次报道CCDC22通过增强IκBα泛素化激活NF-κB信号通路，促进肿瘤细胞侵袭转移，提出其作为潜在治疗靶点。
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**注**：以上文献主题覆盖CCDC22在膜蛋白运输、神经系统疾病和肿瘤中的机制研究，可通过PubMed/Google Scholar输入标题验证原文。如需补充具体期刊名称或PMID可告知。
Coiled-coil domain-containing protein 22 (CCDC22) is a ubiquitously expressed eukaryotic protein characterized by multiple coiled-coil domains, which mediate protein-protein interactions. It plays a regulatory role in intracellular vesicular trafficking and signal transduction, particularly within the endosomal-lysosomal system. CCDC22 interacts with components of the Commander complex (e.g., COMMD proteins) and Rab GTPases, suggesting its involvement in sorting membrane receptors (e.g., EGFR, TLR4) for recycling or degradation.
Mutations in the *CCDC22* gene (located at Xp11.23) are linked to X-linked intellectual disability (XLID) and Ritscher-Schinzel-like syndrome, manifesting neurodevelopmental abnormalities and craniofacial defects. These pathogenic variants disrupt endosomal trafficking, leading to aberrant signaling pathways and impaired neuronal development. Additionally, CCDC22 dysregulation has been implicated in cancer progression, with studies showing altered expression in breast, liver, and colorectal cancers, where it may influence metastasis and chemoresistance.
Despite these findings, the precise molecular mechanisms of CCDC22 remain under investigation. Current research focuses on its scaffolding role in multisubunit complexes and its crosstalk with ubiquitination processes, autophagy, and immune response pathways. Understanding CCDC22’s functions could reveal therapeutic targets for genetic disorders and cancer.
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