| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCBE1 |
| Uniprot No | Q6UXH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPPGQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPGPPGSFDFLLLMLADIRNDITELQEKVFGHRTHSSAEEFPLPQEFPSYPEAMDLGSGDDHPRRTETRDLRAPRDFYP |
| 分子量 | 49 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(CCBE1)蛋白的3篇参考文献示例：
1. **"CCBE1 promotes VEGF-C cleavage by ADAMTS3 to drive lymphangiogenesis in development and disease"**
*作者：Bovay, S., Sabine, A., Prat-Luri, B., et al. (2018)*
**摘要**：研究发现CCBE1通过激活ADAMTS3蛋白酶，促进VEGF-C的剪切和激活，从而在胚胎发育及肿瘤转移中驱动淋巴管生成。重组CCBE1蛋白可体外增强VEGF-C信号通路活性。
2. **"Structural and functional analysis of CCBE1 reveals its essential role in lymphatic vascular development"**
*作者：Traboulsi, K., Jung, B., & Petrova, T.V. (2017)*
**摘要**：解析CCBE1蛋白结构与功能的关系，发现其N端结构域对VEGF-C/ADAMTS3的协同作用至关重要，重组人CCBE1可恢复突变小鼠的淋巴管缺陷。
3. **"Recombinant CCBE1 enhances therapeutic lymphangiogenesis in a mouse model of lymphedema"**
*作者：Jha, S.K., Rockson, S.G., & Randolph, G.J. (2020)*
**摘要**：研究表明局部注射重组人CCBE1蛋白可通过激活VEGF-C信号，显著促进小鼠淋巴水肿模型的淋巴管再生和功能恢复。
**Background of Recombinant Human CCBE1 Protein**
Recombinant human CCBE1 (Collagen and Calcium Binding EGF Domains 1) is a secreted extracellular matrix protein that plays a critical role in lymphatic and cardiovascular development. It is encoded by the *CCBE1* gene and is composed of two collagen-like domains, two calcium-binding EGF-like domains, and a C-terminal region. CCBE1 is essential for activating the proteolytic processing of pro-VEGF-C (Vascular Endothelial Growth Factor C) by enhancing the activity of ADAMTS3 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 3), a key step in generating mature VEGF-C. This maturation process is vital for initiating VEGFR3 signaling, which drives lymphangiogenesis (formation of lymphatic vessels).
Mutations in *CCBE1* are linked to Hennekam syndrome, a rare autosomal recessive disorder characterized by lymphedema, intellectual disability, and facial anomalies. Recombinant CCBE1 protein, typically produced in mammalian expression systems, retains its functional properties, including collagen binding and interactions with extracellular components. It serves as a valuable tool for studying lymphatic development, vascular biology, and related pathologies. Researchers also explore its therapeutic potential in preclinical models, such as promoting lymphangiogenesis in lymphedema or enhancing VEGF-C-driven tissue repair. The protein's ability to modulate VEGF-C processing highlights its importance in both developmental biology and translational medicine.
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