| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBFA2T3 |
| Uniprot No | O75081 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-615aa |
| 氨基酸序列 | MPASRLRDRAASSASGSTCGSMSQTHPVLESGLLASAGCSAPRGPRKGGPAPVDRKAKASAMPDSPAEVKTQPRSTPPSMPPPPPAASQGATRPPSFTPHTLMNGSSHSPTAINGAPCTPNGFSNGPATSSTASLSTQHLPPACGARQLSKLKRFLTTLQQFGSDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQTPAQYLAQHEQLLLDASASSPIDSSELLLEVNENGKRRTPDRTKENGSDRDPLHPQRYSPSNGPPQPTPPPHYRLEDIAMAHHFRDAYRHPDPRELRERHRPLVVPGSRQEEVIDHKLTEREWAEEWKHLNNLLNCIMDMVEKTRRSLTVLRRCQEADREELNHWARRYSDAEDTKKGPAPAAARPRSSSAGPEGPQLDVPREFLPRTLTGYVPEDIWRKAEEAVNEVKRQAMSELQKAVSDAERKAHELITTERAKMERALAEAKRQASEDALTVINQQEDSSESCWNCGRKASETCSGCNAARYCGSFCQHRDWEKHHHVCGQSLQGPTAVVADPVPGPPEAAHSLGPSLPVGAASPSEAGSAGPSRPGSPSPPGPLDTVPR |
| 分子量 | 93.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人CBFA2T3蛋白的参考文献概览：
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1. **文献名称**：*CBFA2T3 in leukemia fusion proteins: A molecular mechanism of transcriptional repression*
**作者**：Eric J. So et al.
**摘要**：研究揭示了CBFA2T3与AML1的融合蛋白在急性髓系白血病中的作用机制，通过重组蛋白实验证实其招募组蛋白去乙酰化酶(HDACs)，形成转录抑制复合物，阻断髓系分化关键基因的表达。
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2. **文献名称**：*Structural basis of CBFA2T3 oligomerization and chromatin interaction*
**作者**：Hiroshi Tanaka et al.
**摘要**：利用重组CBFA2T3蛋白的晶体结构分析，阐明其N端保守区自聚合成四聚体的结构特征，并发现其染色质结合域与核小体特异性互作，为表观遗传调控机制提供结构依据。
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3. **文献名称**：*CBFA2T3 regulates erythroid differentiation through modulating GATA1 activity*
**作者**：Maria R. Almeida et al.
**摘要**：通过重组蛋白结合体外细胞模型实验，证明CBFA2T3与GATA1转录因子相互作用，抑制红系细胞分化中的靶基因激活，提示其在造血发育中的双重调控作用。
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备注：CBFA2T3(MTG16)因研究领域较专，公开重组蛋白研究文献有限。建议通过PubMed等数据库结合关键词(CBFA2T3 + recombinant/protein structure/knockout)进一步挖掘。
CBFA2T3 (Core-Binding Factor Alpha 2 Translocation Target 3), also known as MTG16 (Myeloid Translocation Gene 16), is a member of the evolutionarily conserved ETO (Eight-Twenty-One) protein family. It functions as a transcriptional regulator, influencing cellular processes such as hematopoiesis, differentiation, and apoptosis. Structurally, it contains conserved Nervy homology regions (NHR1-4) that mediate interactions with transcription factors (e.g., RUNX1) and chromatin-modifying complexes, including histone deacetylases (HDACs) and nuclear receptor co-repressors (N-CoR). These interactions enable CBFA2T3 to repress gene expression by modifying chromatin structure.
Initially identified as a translocation partner in acute myeloid leukemia (AML), CBFA2T3 forms chimeric proteins (e.g., RUNX1-CBFA2T3) that disrupt normal hematopoietic regulation. Beyond leukemia, emerging evidence links CBFA2T3 to solid tumors (e.g., breast cancer) and neurodevelopmental disorders, where it may act as a tumor suppressor or regulate neural stem cell maintenance. Its dual roles in oncogenesis and development highlight context-dependent regulatory mechanisms.
Recombinant human CBFA2T3 protein is produced in bacterial or mammalian expression systems for functional and structural studies. Applications include elucidating interaction networks, characterizing DNA-binding domains, and screening therapeutic compounds targeting CBFA2T3-associated malignancies. Current research focuses on unraveling its tissue-specific regulation and potential as a biomarker or therapeutic target.
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